Ivanovska N, Nikolova P, Hristova M, Philipov S, Istatkova R
Institute of Microbiology, Bulgarian Academy of Sciences, Sofia.
Int J Immunopharmacol. 1999 May;21(5):325-36. doi: 10.1016/s0192-0561(99)00014-4.
Eleven bisbenzylisoquinoline alkaloids (BBI) were isolated from the plant Isopyrum thalictroides (L.). Treatment of normal human serum (NHS) with BBI resulted in a diminution of the haemolytic activity of the classical pathway (CP). The mode of action of the main alkaloids isopyruthaline (It1), fangchinoline (It2) and isotalictrine (It3) on CP activation was investigated in vitro. The inhibition was time- and temperature-related and for Itl and It3 depended on the concentration of Ca2+ and Mg2+ ions. It was established that the substances reduced C1 haemolytic activity. It2 and It3 enhanced the complement consumption caused by heat aggregated human IgG (HAGG). The BBI prevented the formation of C3 convertase of the classical pathway. The loss of haemolytic activity was partially restored by the addition of C142 reagent (zymosan-treated guinea pig serum) to alkaloids-treated NHS. The addition of the late components C3-9 (EDTA-treated rat sera) recovered to some extent the haemolytic activity of It1-treated NHS, but not of It2- and It3-treated NHS.
从植物唐松草（Isopyrum thalictroides (L.)）中分离出了11种双苄基异喹啉生物碱（BBI）。用BBI处理正常人血清（NHS）会导致经典途径（CP）的溶血活性降低。体外研究了主要生物碱异唐松草碱（It1）、粉防己碱（It2）和异唐松星碱（It3）对CP激活的作用方式。这种抑制作用与时间和温度有关，对于It1和It3，还取决于Ca2+和Mg2+离子的浓度。已确定这些物质降低了C1溶血活性。It2和It3增强了热聚集人IgG（HAGG）引起的补体消耗。BBI阻止了经典途径C3转化酶的形成。向用生物碱处理的NHS中添加C142试剂（酵母聚糖处理的豚鼠血清）可部分恢复溶血活性的丧失。添加晚期成分C3 - 9（EDTA处理的大鼠血清）在一定程度上恢复了用It1处理的NHS的溶血活性，但不能恢复用It2和It3处理的NHS的溶血活性。
