Chun J, Seong C N, Bae K S, Lee K J, Kang S O, Goodfellow M, Hah Y C
Department of Microbiology, College of Natural Sciences, Seoul National University, Republic of Korea.
Int J Syst Bacteriol. 1998 Jul;48 Pt 3:901-5. doi: 10.1099/00207713-48-3-901.
An actinomycete strain, 'Nocardai flavorosea' JCM 3332, was found to have properties consistent with its classification in the genus Nocardia. An almost complete gene sequence of the 16S rDNA of the strain was determined following cloning and sequencing of the amplified gene. The sequence was aligned with those available for nocardiae and phylogenetic trees were inferred using four tree-making algorithms. The organisms consistently formed a distinct clade with the type strain of Nocardia carnea. However, DNA relatedness experiments showed that the strain and N. carnea DSM 43397T belonged to two distinct genomic species. The organism was also distinguished from representative of all of the validly described species of Nocardia using a combination of phenotypic properties. These genotypic and phenotypic data show that the strain merits recognition as a new species of the genus Nocardia. The name proposed for the new species of is Nocardia flavorosea sp. nov. The type strain is JCM 3332T.
发现一株放线菌菌株“诺卡氏菌flavorosea”JCM 3332具有与其在诺卡氏菌属中的分类相符的特性。在对扩增基因进行克隆和测序后，确定了该菌株16S rDNA的几乎完整的基因序列。将该序列与诺卡氏菌的可用序列进行比对，并使用四种建树算法推断系统发育树。这些生物始终与肉色诺卡氏菌的模式菌株形成一个独特的分支。然而，DNA相关性实验表明，该菌株与肉色诺卡氏菌DSM 43397T属于两个不同的基因组物种。利用一系列表型特性，该生物也与诺卡氏菌所有有效描述物种的代表区分开来。这些基因型和表型数据表明，该菌株值得被认可为诺卡氏菌属的一个新物种。为该新物种提议的名称是诺卡氏菌flavorosea sp. nov.。模式菌株是JCM 3332T。
