用于扩增编码细菌16S rRNA基因的有用细菌特异性PCR引物的设计与评估。
Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA.
作者信息
Marchesi J R, Sato T, Weightman A J, Martin T A, Fry J C, Hiom S J, Dymock D, Wade W G
机构信息
School of Pure and Applied Biology, University of Wales College of Cardiff, United Kingdom.
出版信息
Appl Environ Microbiol. 1998 Feb;64(2):795-9. doi: 10.1128/AEM.64.2.795-799.1998.
Abstract
We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecological and systematic studies than PCR amplimers that are currently more generally used.
摘要
我们报告了用于从细菌中扩增16S rRNA基因的PCR引物63f和1387r的设计与评估。利用一种细菌物种和环境样本对它们的特异性和有效性进行了系统测试。结果发现，在生态学和系统学研究中，它们比目前更常用的PCR扩增引物在16S rRNA基因扩增方面更有用。
相似文献
1
Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA.
Appl Environ Microbiol. 1998 Feb;64(2):795-9. doi: 10.1128/AEM.64.2.795-799.1998.
2
Review and re-analysis of domain-specific 16S primers.
J Microbiol Methods. 2003 Dec;55(3):541-55. doi: 10.1016/j.mimet.2003.08.009.
3
Improved PCR primers to amplify 16S rRNA genes from NC10 bacteria.
Appl Microbiol Biotechnol. 2016 Jun;100(11):5099-108. doi: 10.1007/s00253-016-7477-9. Epub 2016 Mar 28.
4
The 16s/23s ribosomal spacer region as a target for DNA probes to identify eubacteria.
PCR Methods Appl. 1991 Aug;1(1):51-6. doi: 10.1101/gr.1.1.51.
5
Errors in ribosomal sequence datasets generated using PCR-coupled 'panbacterial' pyrosequencing, and the establishment of an improved approach.
Mol Cell Probes. 2013 Feb;27(1):65-7. doi: 10.1016/j.mcp.2012.07.003. Epub 2012 Jul 20.
6
Cross-kingdom amplification using bacteria-specific primers: complications for studies of coral microbial ecology.
Appl Environ Microbiol. 2008 Dec;74(24):7828-31. doi: 10.1128/AEM.01303-08. Epub 2008 Oct 17.
7
Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis.
FEMS Microbiol Ecol. 2007 May;60(2):341-50. doi: 10.1111/j.1574-6941.2007.00283.x. Epub 2007 Mar 2.
8
Detection and identification of bacteria in clinical samples by 16S rRNA gene sequencing: comparison of two different approaches in clinical practice.
J Med Microbiol. 2012 Apr;61(Pt 4):483-488. doi: 10.1099/jmm.0.030387-0. Epub 2011 Dec 8.
9
Detection of Verrucomicrobia in a pasture soil by PCR-mediated amplification of 16S rRNA genes.
Appl Environ Microbiol. 1999 Sep;65(9):4280-4. doi: 10.1128/AEM.65.9.4280-4284.1999.
10
Touchdown enzyme time release-PCR for detection and identification of Chlamydia trachomatis, C. pneumoniae, and C. psittaci using the 16S and 16S-23S spacer rRNA genes.
J Clin Microbiol. 2000 Mar;38(3):1085-93. doi: 10.1128/JCM.38.3.1085-1093.2000.
引用本文的文献
1
Cataloging variation in 16S rRNA gene sequences of female urobiome bacteria.
Front Urol. 2024 Jan 8;3:1270509. doi: 10.3389/fruro.2023.1270509. eCollection 2023.
2
Treatment with mitochondrial targeting antibiotics improves survival outcomes after Flock House virus infection in young and aged Drosophila melanogaster.
Res Sq. 2025 Jun 10:rs.3.rs-6816306. doi: 10.21203/rs.3.rs-6816306/v1.
3
Novel Halotolerant Bacteria from Saline Environments: Isolation and Biomolecule Production.
BioTech (Basel). 2025 Jun 19;14(2):49. doi: 10.3390/biotech14020049.
4
Probiotic potential of bacteria associated with the mangrove epiphytic algae and .
PeerJ. 2025 Jun 3;13:e19073. doi: 10.7717/peerj.19073. eCollection 2025.
5
Sheep challenged with sheep-derived type II Mycobacterium avium subsp. paratuberculosis: the first experimental model of paratuberculosis in China.
BMC Vet Res. 2025 Apr 29;21(1):298. doi: 10.1186/s12917-025-04765-1.
6
Investigating the Potential of Native Soil Bacteria for Diesel Biodegradation.
Microorganisms. 2025 Mar 2;13(3):564. doi: 10.3390/microorganisms13030564.
7
Bacterial diversity in the honey sac during bee foraging on winter-flowering flora and dominant inhibits .
Front Insect Sci. 2025 Mar 11;5:1555434. doi: 10.3389/finsc.2025.1555434. eCollection 2025.
8
Photofermentative production of poly-β-hydroxybutyrate (PHB) by purple non-sulfur bacteria using olive oil by-products.
Bioresour Bioprocess. 2025 Mar 24;12(1):25. doi: 10.1186/s40643-025-00856-x.
9
Spontaneous lung colonization in the cystic fibrosis rat model is linked to gastrointestinal obstruction.
mBio. 2025 Apr 9;16(4):e0388324. doi: 10.1128/mbio.03883-24. Epub 2025 Mar 5.
10
Predatory potentials of novel isolates against multidrug-resistant and extremely drug-resistant bacterial pathogens of animals and plants.
3 Biotech. 2025 Mar;15(3):69. doi: 10.1007/s13205-025-04230-8. Epub 2025 Feb 27.
本文引用的文献
1
Database on the structure of small ribosomal subunit RNA.
Nucleic Acids Res. 1997 Jan 1;25(1):111-6. doi: 10.1093/nar/25.1.111.
2
A computer analysis of primer and probe hybridization potential with bacterial small-subunit rRNA sequences.
Appl Environ Microbiol. 1996 Mar;62(3):872-9. doi: 10.1128/aem.62.3.872-879.1996.
3
The oligonucleotide probe database.
Appl Environ Microbiol. 1996 Oct;62(10):3557-9. doi: 10.1128/aem.62.10.3557-3559.1996.
4
Phylogenetic identification and in situ detection of individual microbial cells without cultivation.
Microbiol Rev. 1995 Mar;59(1):143-69. doi: 10.1128/mr.59.1.143-169.1995.
5
Phylogenetic analysis of the hyperthermophilic pink filament community in Octopus Spring, Yellowstone National Park.
Appl Environ Microbiol. 1994 Jun;60(6):2113-9. doi: 10.1128/aem.60.6.2113-2119.1994.
6
Bacterial evolution.
Microbiol Rev. 1987 Jun;51(2):221-71. doi: 10.1128/mr.51.2.221-271.1987.
7
Archaea in coastal marine environments.
Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5685-9. doi: 10.1073/pnas.89.12.5685.
8
DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities.
FEMS Microbiol Lett. 1992 Dec 15;100(1-3):59-65. doi: 10.1111/j.1574-6968.1992.tb14019.x.
9
Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801-5. doi: 10.1073/pnas.75.10.4801.
Zotero 插件