Jones C N, Grant L D, Prange A J
Psychopharmacology (Berl). 1978 Dec 8;59(3):217-24. doi: 10.1007/BF00426625.
Male Sprague-Dawley rats were trained in a two-lever operant discrimination task using 20 mg/kg thyrotropin-releasing hormone (TRH) and saline as cues. Following completion of 40 daily training sessions, 22 of 25 subjects demonstrated a high level of discriminative responding based on the TRH and saline cues. An evaluation of the time course of TRH indicated that the stimulus properties peak between 5 and 15 min and dissipate substantially by 55--65 min. During additional testing, rats showed dose-dependent generalization between the training treatments (20 mg/kg TRH and saline) and novel doses of TRH (1, 5, 10, and 40 MG/KG). However, animals failed to show generalization between the training drug (20 mg/kg TRH) and d-amphetamine sulfate (0.8, 1.6, or 2.4 mg/kg); likewise, animals trained to discriminate d-amphetamine (0.8 or 1.6 mg/kg) from saline failed to show generalization between d-amphetamine and TRH (10, 20, OR 30 MG/KG). Microgram quanitites of TRH (2.5--25 microgram administered into either the lateral or third ventricle elicited dose-dependent generalization to the training drug (TRH 20 mg/kg, i.p.), suggesting a CNS mechanism of action for this effect of TRH.
将雄性斯普拉格-道利大鼠置于双杠杆操作性辨别任务中进行训练，使用20毫克/千克促甲状腺激素释放激素（TRH）和生理盐水作为线索。在完成40次每日训练后，25只受试大鼠中有22只基于TRH和生理盐水线索表现出高水平的辨别反应。对TRH时间进程的评估表明，其刺激特性在5至15分钟达到峰值，并在55 - 65分钟时大幅消散。在额外测试中，大鼠在训练处理（20毫克/千克TRH和生理盐水）与新剂量的TRH（1、5、10和40毫克/千克）之间表现出剂量依赖性泛化。然而，动物在训练药物（20毫克/千克TRH）和硫酸右苯丙胺（0.8、1.6或2.4毫克/千克）之间未表现出泛化；同样，经训练从生理盐水中辨别出右苯丙胺（0.8或1.6毫克/千克）的动物在右苯丙胺和TRH（10、20或30毫克/千克）之间也未表现出泛化。将微克量的TRH（2.5 - 25微克）注入侧脑室或第三脑室会引发对训练药物（20毫克/千克TRH，腹腔注射）的剂量依赖性泛化，这表明TRH这种作用存在中枢神经系统作用机制。
