López-Carrillo L, Torres-Sánchez L, López-Cervantes M, Blair A, Cebrián M E, Uribe M
National Institute of Public Health, Mexico Secretariat of Health, Av. Universidad No. 655 Col. Sta. María Ahuacatitlán, Morelos, Cuernavaca, México.
Environ Res. 1999 Aug;81(2):142-5. doi: 10.1006/enrs.1999.3961.
Dichlorodiphenyldichloroethane (DDE) adipose tissue level has been regarded as a preferred indicator of accumulated human exposure to DDT; however, blood sera are more feasible to obtain and analyze than adipose tissue samples. Inconsistent and scarce information exists in relation to the adipose tissue/serum DDE ratio. As a part of a hospital-based case-control study performed in Mexico City from 1994 to 1996, 198 paired serum and adipose tissue samples were obtained from 72 women with histologically confirmed breast cancer and 126 women with benign breast disease. Both adipose tissue and serum DDE levels were determined by gas-liquid chromatography and reported as ppb lipid weight (ng/g) as well as wet basis (ng/ml). Results showed that the adipose tissue/serum DDE ratio (ADSE) varies according to the type of information (lipid vs wet basis, arithmetic vs geometric means) used for its estimation. ADSE gets a value near 1 (1.1) only when the geometric DDE levels in lipid basis are used for its estimation. The correlation between DDE serum and adipose tissue levels was found (r=0.364, P<0.001). The ADSE did not vary by disease status, nor was it altered by parity, history of breast-feeding, and other reproductive characteristics. We endorse the use of venipuncture instead of biopsy as a way to estimate DDT body burden levels in further research.
二氯二苯二氯乙烷（DDE）的脂肪组织水平一直被视为人类累积接触滴滴涕（DDT）的首选指标；然而，血清比脂肪组织样本更易于获取和分析。关于脂肪组织/血清DDE比值的信息并不一致且稀缺。作为1994年至1996年在墨西哥城开展的一项基于医院的病例对照研究的一部分，从72例经组织学确诊为乳腺癌的女性和126例患有乳腺良性疾病的女性中获取了198对血清和脂肪组织样本。通过气液色谱法测定脂肪组织和血清中的DDE水平，并以每克脂质重量的微克数（ng/g）以及湿重基础（ng/ml）进行报告。结果表明，脂肪组织/血清DDE比值（ADSE）会因用于估算的信息类型（脂质与湿重基础、算术平均值与几何平均值）而有所不同。仅当使用脂质基础的几何DDE水平进行估算时，ADSE才会接近1（1.1）。发现DDE血清水平与脂肪组织水平之间存在相关性（r = 0.364，P < 0.001）。ADSE不因疾病状态而变化，也不受产次、母乳喂养史及其他生殖特征的影响。我们支持在进一步研究中采用静脉穿刺而非活检来估算DDT的体内负荷水平。
