Winzeler E A, Shoemaker D D, Astromoff A, Liang H, Anderson K, Andre B, Bangham R, Benito R, Boeke J D, Bussey H, Chu A M, Connelly C, Davis K, Dietrich F, Dow S W, El Bakkoury M, Foury F, Friend S H, Gentalen E, Giaever G, Hegemann J H, Jones T, Laub M, Liao H, Liebundguth N, Lockhart D J, Lucau-Danila A, Lussier M, M'Rabet N, Menard P, Mittmann M, Pai C, Rebischung C, Revuelta J L, Riles L, Roberts C J, Ross-MacDonald P, Scherens B, Snyder M, Sookhai-Mahadeo S, Storms R K, Véronneau S, Voet M, Volckaert G, Ward T R, Wysocki R, Yen G S, Yu K, Zimmermann K, Philippsen P, Johnston M, Davis R W
Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305-5307, USA.
Science. 1999 Aug 6;285(5429):901-6. doi: 10.1126/science.285.5429.901.
The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.
在基因组测序项目中鉴定出的许多开放阅读框（ORF）的功能尚不清楚。需要新的全基因组方法来系统地确定它们的功能。通过高通量策略构建了总共6925株酿酒酵母菌株，每株精确缺失2026个ORF中的一个（占基因组中ORF的三分之一以上）。在缺失的ORF中，17%对在丰富培养基中的生存能力至关重要。同时对500多个缺失菌株的表型进行了测定。在缺失菌株中，40%在丰富或基本培养基中表现出定量生长缺陷。
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