Miyamoto Y, Itoh K
Laboratory of Veterinary Public Health, Graduate School of Agriculture and Life Science, University of Tokyo, Japan.
FEMS Microbiol Lett. 1999 Aug 1;177(1):143-9. doi: 10.1111/j.1574-6968.1999.tb13725.x.
To develop a new simple method for identification of bacteria in the Bacteroides subgroup isolated from human feces, we designed a panel of four 16S rDNA-targeted oligonucleotide probes specific for each cluster of the Bacteroides subgroup. The probes Bac and bacvul were targeted to the Bacteroides cluster, and the probes Pre and Por have their target regions characteristic to those of the Prevotella cluster and the Porphyromonas cluster, respectively. The probes presented in this work were constructed to be specific for reference strains in each cluster of the Bacteroides subgroup and were not cross-hybridized with other major intestinal bacteria. The use of combination of these four probes will faciliate the identification of the clusters of the Bacteroides subgroup harbored in the intestine as compared to biological and biochemical testings.
为开发一种用于鉴定从人类粪便中分离出的拟杆菌亚群细菌的新的简单方法，我们设计了一组针对拟杆菌亚群每个簇的四个16S rDNA靶向寡核苷酸探针。探针Bac和bacvul靶向拟杆菌簇，而探针Pre和Por的靶区域分别具有普雷沃菌簇和卟啉单胞菌簇的特征。本研究中提出的探针构建为对拟杆菌亚群每个簇中的参考菌株具有特异性，并且不与其他主要肠道细菌交叉杂交。与生物学和生化检测相比，使用这四种探针的组合将有助于鉴定肠道中存在的拟杆菌亚群的簇。
