Goodgame R, Stager C, Marcantel B, Alcocer E, Segura A M
Baylor College of Medicine, Medicine-Gastroenterology, Houston, TX 77030, USA.
J Infect Dis. 1999 Sep;180(3):929-32. doi: 10.1086/314914.
To quantify intensity of infection in AIDS-related microsporidiosis, 20 patients with known microsporidiosis submitted stools for quantitative spore counts after staining with a calcofluor white stain. Nine patients collected stools for 24 h, for assessment of daily spore excretion, stool-to-stool variation in spore excretion, and patient-to-patient variation in intensity of infection. The number of organisms seen in small bowel biopsy specimens from 7 patients was compared with quantitative fecal spore excretion. Fecal spore concentration in 20 patients ranged from 4.5x105 to 4.4x108 spores/mL of stool. There was a strong correlation between fecal spore excretion and duodenal biopsy spore counts (r=.82; P<.024). Microsporidium infections in AIDS patients can be quantified by counting spores in stool and by small bowel biopsy. Variations in intensity of infection from patient to patient are great and are similar to those in AIDS-related Cryptosporidium infection.
为了量化艾滋病相关微孢子虫病的感染强度，20例已知患有微孢子虫病的患者在经荧光增白剂染色后提交粪便样本进行孢子定量计数。9例患者收集粪便24小时，以评估每日孢子排泄量、粪便间孢子排泄差异以及患者间感染强度差异。将7例患者小肠活检标本中观察到的病原体数量与粪便孢子定量排泄进行比较。20例患者的粪便孢子浓度范围为每毫升粪便4.5×10⁵至4.4×10⁸个孢子。粪便孢子排泄与十二指肠活检孢子计数之间存在强相关性（r = 0.82；P < 0.024）。艾滋病患者的微孢子虫感染可通过粪便孢子计数和小肠活检进行量化。患者间感染强度差异很大，与艾滋病相关隐孢子虫感染的差异相似。
