Abreu P M, Martins E S, Kayser O, Bindseil K U, Siems K, Seemann A, Frevert J
Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Portugal.
Phytomedicine. 1999 Jul;6(3):187-95. doi: 10.1016/S0944-7113(99)80008-7.
Following an ethnobotanical search carried out in Guinea-Bissau, eighteen extracts derived from sixteen medicinal species were screened for antimicrobial, antitumor and antileishmania activity. Significant antitumor activity was found for Holarrhena floribunda against KB (squamous carcinoma), SK-Mel 28 (melanoma), A 549 (lung carcinoma) and MDA-MB 231 (mamma carcinoma) cell lines, with corresponding IC50 values of 7.9, 9.0, 3.4 and 9.9 micrograms/ml. Khaya senegalensis and Anthostema senegalense exhibited a significant activity against Leishmania donovani with IC50 values of 9.8 and 9.1 micrograms/ml, respectively. Most of the extracts showed week or moderate antibacterial and antifungal activity, with MIC values in the range 0.25-1.0 mg/ml. Active extracts were submitted to bioassay-guided fractionation, and the IC50 and MIC of the active fractions were determined.
在几内亚比绍进行了一次民族植物学调查后，对从16种药用植物中提取的18种提取物进行了抗菌、抗肿瘤和抗利什曼原虫活性筛选。发现多花止泻木对KB（鳞状细胞癌）、SK-Mel 28（黑色素瘤）、A 549（肺癌）和MDA-MB 231（乳腺癌）细胞系具有显著的抗肿瘤活性，相应的IC50值分别为7.9、9.0、3.4和9.9微克/毫升。塞内加尔桃花心木和塞内加尔花锚对杜氏利什曼原虫表现出显著活性，IC50值分别为9.8和9.1微克/毫升。大多数提取物显示出较弱或中等的抗菌和抗真菌活性，MIC值在0.25-1.0毫克/毫升范围内。对活性提取物进行了生物测定导向的分级分离，并测定了活性级分的IC50和MIC。
