Robertson J, Murdoch S, Foster L, Green S
Lyme Disease Reference Unit, Public Health Laboratory, Southampton General Hospital, UK.
Eur J Epidemiol. 1999 May;15(5):499-500. doi: 10.1023/a:1007591501411.
Skin biopsies taken from UK cases of erythema migrans rash were cultured for Borrelia burgdorferi sensu lato. Reverse line blotting was used to type the infecting genospecies in PCR-positive cultures and biopsies. B. garinii or B. afzelii was identified in 56% (5/9) of biopsies/cultures tested. All patients were tested by conventional serology. PCR confirmed infection in two patients where serological testing failed to detect antibody.
从英国莱姆病游走性红斑皮疹病例中采集皮肤活检样本，用于培养伯氏疏螺旋体狭义种。采用反向线印迹法对PCR阳性培养物和活检样本中的感染基因种进行分型。在56%（5/9）的检测活检样本/培养物中鉴定出伽氏疏螺旋体或阿氏疏螺旋体。所有患者均接受传统血清学检测。在两名血清学检测未能检测到抗体的患者中，PCR证实了感染。
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