Bourne K Z, Bourne N, Reising S F, Stanberry L R
Children's Hospital Research Foundation, Department of Pediatrics, University of Cincinnati College of Medicine, OH 45229-3039, USA.
Antiviral Res. 1999 Jul;42(3):219-26. doi: 10.1016/s0166-3542(99)00020-0.
There is considerable interest in developing topical microbicides; products to be used intravaginally by women for protection against sexually transmitted diseases. Many compounds derived from plants have been shown to have antimicrobial properties. We examined 19 such compounds in vitro by plaque reduction assay to determine their activity against a common sexually transmitted pathogen, herpes simplex virus type 2. Compounds with an ED50 < or = 7.0 mg/ml were tested for efficacy in vivo. Four compounds, carrageenan lambda type IV, cineole, curcumin, and eugenol, provided significant protection (P < 0.05) in a mouse model of intravaginal HSV-2 challenge. Eugenol, which provided the greatest protection in mice was also evaluated using the guinea pig model of genital HSV-2 infection where it also demonstrated significant protection. Based on these results, several plant-derived compounds appear to warrant further evaluation as potential microbicides.
开发局部用杀微生物剂有很大的研究兴趣；这类产品供女性阴道内使用，用于预防性传播疾病。许多源自植物的化合物已被证明具有抗菌特性。我们通过噬斑减少试验对19种此类化合物进行了体外研究，以确定它们对一种常见的性传播病原体——2型单纯疱疹病毒的活性。对半数有效浓度（ED50）≤7.0毫克/毫升的化合物进行了体内疗效测试。四种化合物，即IV型卡拉胶、桉叶油素、姜黄素和丁香酚，在阴道内接种HSV-2的小鼠模型中提供了显著的保护作用（P<0.05）。在小鼠中提供最大保护作用的丁香酚，也在豚鼠生殖器HSV-2感染模型中进行了评估，结果它同样显示出显著的保护作用。基于这些结果，几种源自植物的化合物似乎有必要作为潜在的杀微生物剂作进一步评估。
