Logothetis N K, Guggenberger H, Peled S, Pauls J
Max Planck Institute for Biological Cybernetics, Spemannstr. 38, 72076 Tübingen, Germany.
Nat Neurosci. 1999 Jun;2(6):555-62. doi: 10.1038/9210.
Functional magnetic resonance imaging (fMRI) has become an essential tool for studying human brain function. Here we describe the application of this technique to anesthetized monkeys. We present spatially resolved functional images of the monkey cortex based on blood oxygenation level dependent (BOLD) contrast. Checkerboard patterns or pictures of primates were used to study stimulus-induced activation of the visual cortex, in a 4.7-Tesla magnetic field, using optimized multi-slice, gradient-recalled, echo-planar imaging (EPI) sequences to image the entire brain. Under our anesthesia protocol, visual stimulation yielded robust, reproducible, focal activation of the lateral geniculate nucleus (LGN), the primary visual area (V1) and a number of extrastriate visual areas, including areas in the superior temporal sulcus. Similar responses were obtained in alert, behaving monkeys performing a discrimination task.
功能磁共振成像（fMRI）已成为研究人类大脑功能的重要工具。在此，我们描述了该技术在麻醉猴身上的应用。我们基于血氧水平依赖（BOLD）对比呈现了猴皮层的空间分辨功能图像。在4.7特斯拉磁场中，使用优化的多层梯度回波平面成像（EPI）序列对整个大脑进行成像，采用棋盘格图案或灵长类动物图片来研究视觉皮层的刺激诱发激活。在我们的麻醉方案下，视觉刺激在外侧膝状体（LGN）、初级视觉区（V1）以及包括颞上沟区域在内的多个纹外视觉区产生了强烈、可重复的局灶性激活。在执行辨别任务的清醒行为猴中也获得了类似的反应。
