Arvaja M, Lehtinen M, Koskela P, Lappalainen M, Paavonen J, Vesikari T
Department of Virology and Vaccine Research, University of Tampere Medical School, Finland.
Sex Transm Infect. 1999 Jun;75(3):168-71. doi: 10.1136/sti.75.3.168.
Serological evaluation of herpes simplex virus infections during pregnancy.
2991 serum samples were obtained during 1st, 2nd, and 3rd trimester from 997 pregnant women. Baculovirus expressed glycoproteins gG1 (HSV-1) and gG2 (HSV-2) were used as antigens in ELISA for HSV-1 and HSV-2 IgG and IgA antibodies.
The prevalence of HSV-1 gG1 antibodies was 70% and that of HSV-2 gG2 antibodies 16%. Among susceptible women we found five (0.6%) cases with serological evidence of primary HSV-2 infection during pregnancy. Evidence of active HSV-1 infection was found in nine (0.9%) cases. Decline of HSV-2 gG2 IgG antibody levels during pregnancy was pronounced compared with HSV-1 gG1 IgG antibody levels (p < 0.01); also the proportion of seroreversions was considerably higher among HSV-2 seropositives (25%) than among HSV-1 seropositives (3%) (p < 0.001).
HSV-2 gG2 IgG antibodies were readily distinguished from HSV-1 gG1 IgG antibodies by the glycoprotein gG ELISAs. Serological assays for gG2 antibodies should guard against the decline of specific antibodies during pregnancy.
孕期单纯疱疹病毒感染的血清学评估。
从997名孕妇的孕早期、孕中期和孕晚期获取了2991份血清样本。用杆状病毒表达的糖蛋白gG1（HSV-1）和gG2（HSV-2）作为抗原，通过ELISA检测HSV-1和HSV-2的IgG和IgA抗体。
HSV-1 gG1抗体的阳性率为70%，HSV-2 gG2抗体的阳性率为16%。在易感女性中，我们发现5例（0.6%）在孕期有原发性HSV-2感染的血清学证据。9例（0.9%）有活动性HSV-1感染的证据。与HSV-1 gG1 IgG抗体水平相比，孕期HSV-2 gG2 IgG抗体水平明显下降（p<0.01）；HSV-2血清阳性者中血清学逆转的比例（25%）也明显高于HSV-1血清阳性者（3%）（p<0.001）。
通过糖蛋白gG ELISA可轻易区分HSV-2 gG2 IgG抗体和HSV-1 gG1 IgG抗体。gG2抗体的血清学检测应注意孕期特异性抗体的下降。
