Wilkerson M, McAllister S, Miller J M, Heiter B J, Bourbeau P P
Division of Laboratory Medicine, Geisinger Medical Center, Danville, Pennsylvania 17822, USA.
J Clin Microbiol. 1997 Jan;35(1):148-51. doi: 10.1128/jcm.35.1.148-151.1997.
Various commercially produced agglutination kits are widely used for the identification of Staphylococcus aureus. These kits detect the presence of protein A and/or clumping factor on S. aureus. The literature has shown that methicillin-resistant S. aureus (MRSA) isolates which are deficient in both clumping factor and protein A may be misidentified. Two products, Slidex and Staphaurex Plus, utilize specific anti-S. aureus antibodies, potentially giving them greater sensitivity compared to products without these antibodies. We report a prospective study designed to compare the performance characteristics of Fastaph, Slidex, Staphaurex, Staphaurex Plus, Staphyloslide, and the tube coagulase test for the identification of staphylococcal isolates. All discrepant isolates were tested with the Gen-Probe AccuProbe S. aureus test and were identified to the species level with conventional reference biochemicals. A total of 1,193 isolates were tested, including 33 MRSA and 423 methicillin-sensitive S. aureus isolates. The sensitivities and specificities of the tests, respectively, were as follows: Fastaph, 99.1 and 98.9%; Slidex, 99.6 and 96.4%; Staphaurex, 98.9 and 99.9%; Staphaurex Plus, 99.6 and 93.9%; Staphyloslide, 99.1 and 98.9%; and tube coagulase, 99.3 and 100%. Sensitivity was excellent for all of the products tested. The specificities of Fastaph, Staphaurex, and Staphyloslide were excellent, while Staphaurex Plus and Slidex demonstrated less optimal results.
各种商业生产的凝集试剂盒被广泛用于金黄色葡萄球菌的鉴定。这些试剂盒可检测金黄色葡萄球菌上蛋白A和/或凝聚因子的存在。文献表明，同时缺乏凝聚因子和蛋白A的耐甲氧西林金黄色葡萄球菌（MRSA）分离株可能会被错误鉴定。两种产品，即Slidex和Staphaurex Plus，利用特异性抗金黄色葡萄球菌抗体，与没有这些抗体的产品相比，可能具有更高的灵敏度。我们报告了一项前瞻性研究，旨在比较Fastaph、Slidex、Staphaurex、Staphaurex Plus、Staphyloslide以及试管凝固酶试验在鉴定葡萄球菌分离株方面的性能特征。所有有差异的分离株都用Gen-Probe AccuProbe金黄色葡萄球菌试验进行检测，并通过传统的参考生化方法鉴定到种水平。总共检测了1193株分离株，包括33株MRSA和423株甲氧西林敏感金黄色葡萄球菌分离株。这些试验的灵敏度和特异性分别如下：Fastaph为99.1%和98.9%；Slidex为99.6%和96.4%；Staphaurex为98.9%和99.9%；Staphaurex Plus为99.6%和93.9%；Staphyloslide为99.1%和98.9%；试管凝固酶为99.3%和100%。所有测试产品的灵敏度都很好。Fastaph、Staphaurex和Staphyloslide的特异性很好，而Staphaurex Plus和Slidex的结果不太理想。
