Jensen A T, Gaafar A, Ismail A, Christensen C B, Kemp M, Hassan A M, Kharazmi A, Theander T G
Center for Medical Parasitology, University of Copenhagen, Denmark.
Am J Trop Med Hyg. 1996 Nov;55(5):490-5. doi: 10.4269/ajtmh.1996.55.490.
An enzyme-linked immunosorbent assay (ELISA) using a 28 amino acid sequence of the repetitive element of gene B protein (GBP) from Leishmania major was developed for serodiagnosis of cutaneous leishmaniasis (CL). The assay was compared to ELISAs using crude amastigote and promastigote antigens from L. donovani and the major surface glycoprotein (Gp63) from either L. donovani or L. major as a solid-phase ligand. The sensitivity of the assays was tested in 33 patients suffering from CL caused by L. major. The sensitivity of the GBP peptide (GBPP) ELISA was 82%. This was higher than in the assays using crude amastigote (67%) or promastigote (67%) antigens, but the difference was not statistically significant. The sensitivity in the assays using Gp63 from L. donovani (52%) or L. major (39%) was significantly lower than in the assay using GBPP (P = 0.019 and P < 0.001, respectively). Plasma samples from healthy Sudanese individuals living in an area endemic for malaria but free of leish-maniasis were negative in all the assays. Significantly higher levels of antibodies were found in the patients who had suffered from the disease for more than eight weeks than in patients with a shorter clinical history (GBPP ELISA; P = 0.038; amastigote ELISA; P = 0.004; and promastigote ELISA; P = 0.017). In the former group, the sensitivities of the five ELISAs were 100% (GBPP), 87% (amastigote), 93% (promastigote), 67% (L. donovani), and 53% (L. major), respectively.
利用来自硕大利什曼原虫基因B蛋白（GBP）重复元件的28个氨基酸序列开发了一种酶联免疫吸附测定（ELISA），用于皮肤利什曼病（CL）的血清学诊断。将该测定与使用来自杜氏利什曼原虫的粗制无鞭毛体和前鞭毛体抗原以及来自杜氏利什曼原虫或硕大利什曼原虫的主要表面糖蛋白（Gp63）作为固相配体的ELISA进行比较。在33例由硕大利什曼原虫引起的CL患者中测试了这些测定的敏感性。GBP肽（GBPP）ELISA的敏感性为82%。这高于使用粗制无鞭毛体（67%）或前鞭毛体（67%）抗原的测定，但差异无统计学意义。使用来自杜氏利什曼原虫（52%）或硕大利什曼原虫（39%）的Gp63的测定的敏感性明显低于使用GBPP的测定（分别为P = 0.019和P < 0.001）。生活在疟疾流行但无利什曼病地区的健康苏丹个体的血浆样本在所有测定中均为阴性。病程超过8周的患者体内抗体水平明显高于病程较短的患者（GBPP ELISA；P = 0.038；无鞭毛体ELISA；P = 0.004；前鞭毛体ELISA；P = 0.017）。在前一组中，五种ELISA的敏感性分别为100%（GBPP）、87%（无鞭毛体）、93%（前鞭毛体）、67%（杜氏利什曼原虫）和53%（硕大利什曼原虫）。
