Murohashi T, Yoshida K
Acta Leprol. 1978 Jan-Mar(70):23-33.
M-Y 16j agar slant was prepared by modifying M-Y 14b which has hitherto been used most widely in our experiments by increasing the amount of Na pantothenate and adding leucine, and the growth stimulating effect was investigated referring to the foregoing subculture experiments. The results revealed that the growth of M. leprae was stimulated remarkably in the primary isolation quite similarly to the subculture. This seemed to be resulted from stimulated biosynthesis of fatty acids by the leucine metabolism. The authors are greatly indebted to the World Health Organization and the Comité Exécutif International pour l'Assistance aux Lépreux for their financial support and to Dr. S. Ishihara, the Director of the National Suruga Leprosarium, for his kind supply of the materials to be cultured and for the lepromin test in leprosy patients carried out by himself. The main point of this study was presented to the 50th Annual Meeting of the Japanese Leprosy Association.
M-Y 16j琼脂斜面是通过对我们实验中迄今使用最广泛的M-Y 14b进行改良制备的，增加了泛酸钠的量并添加了亮氨酸，并参照上述传代培养实验研究了其生长刺激作用。结果表明，在初次分离中，麻风杆菌的生长受到显著刺激，与传代培养非常相似。这似乎是由亮氨酸代谢刺激脂肪酸生物合成所致。作者非常感谢世界卫生组织和国际援助麻风病人执行委员会的资金支持，以及静冈国立麻风病疗养院院长石原博士，感谢他慷慨提供待培养的材料以及他亲自为麻风病人进行的麻风菌素试验。本研究的要点已在日本麻风病协会第50届年会上发表。
