大肠杆菌中化学感受器复合体的聚集独立于甲基转移酶CheR和甲酯酶CheB。
Clustering of the chemoreceptor complex in Escherichia coli is independent of the methyltransferase CheR and the methylesterase CheB.
作者信息
Lybarger S R, Maddock J R
机构信息
Department of Biology, University of Michigan, Ann Arbor, Michigan 48109-1048, USA.
出版信息
J Bacteriol. 1999 Sep;181(17):5527-9. doi: 10.1128/JB.181.17.5527-5529.1999.
Abstract
The Escherichia coli chemoreceptors and their associated cytoplasmic proteins, CheA and CheW, cluster predominantly at the cell poles. The nature of the clustering remains a mystery. Recent studies suggest that CheR binding to and/or methylation of the chemoreceptors may play a role in chemoreceptor complex aggregation. In this study, we examined the intracellular distribution of the chemoreceptors by immunoelectron microscopy in strains lacking either the methyltransferase CheR or the methylesterase CheB. The localization data revealed that, in vivo, aggregation of the chemoreceptor complex was independent of either CheR or CheB.
摘要
大肠杆菌化学感受器及其相关的胞质蛋白CheA和CheW主要聚集在细胞两极。这种聚集的性质仍是个谜。最近的研究表明，CheR与化学感受器的结合和/或甲基化可能在化学感受器复合物聚集中起作用。在本研究中，我们通过免疫电子显微镜检查了缺乏甲基转移酶CheR或甲酯酶CheB的菌株中化学感受器的细胞内分布。定位数据显示，在体内，化学感受器复合物的聚集与CheR或CheB无关。
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