Andréoletti L, Dubois V, Lescieux A, Dewilde A, Bocket L, Fleury H J, Wattré P
Laboratoire de Virologie, Lille, France.
AIDS. 1999 Aug 20;13(12):1469-75. doi: 10.1097/00002030-199908200-00005.
Human polyomavirus JC (JCV) induces human progressive multifocal leukoencephalopathy (PML) in patients with AIDS. Peripheral blood mononuclear cells (PBMC) of HIV-1-positive immunocompetent and immunocompromised patients can harbour JCV genome, but their precise role in JCV latency or reactivation status before the onset of PML remains hypothetical.
To assess JCV latency or reactivation status in PBMC of HIV-1-positive immunocompromised patients without PML.
A group of 82 HIV-1-positive immunocompromised patients who did not have PML were compared with 10 patients with AIDS and PML and with 69 HIV-1-positive immunocompetent patients without PML.
DNA and total RNA were extracted from PBMC. The presence of JCV DNA was demonstrated by a semi-nested polymerase chain reaction (PCR). By using primer pairs specific for an early gene,T, and a late gene, VP1, the expression of both early and late gene mRNA in PBMC could be identified using reverse transcriptase (RT) PCR.
JCV DNA was detected by PCR in 17.4% of 69 HIV-1-positive immunocompetent patients, in 23.2% of 82 HIV-1-positive immunocompromised patients, and in 60% of 10 patients with AIDS and PML. No correlation could be drawn between the detection of JCV DNA in the PBMC and the clinical or biological status of the HIV-1-positive patients. By using RT-PCR procedures, no expression of JCV early and late mRNA in PBMC was found in any patients.
JCV DNA is detectable in the PBMC of 20.5% of 151 HIV-1-infected patients independently of the CDC (Centers for Disease Control and Prevention) stages of the infection. Moreover, our results suggest that active replication of JCV in PBMC appears to be absent or at least a very rare event in HIV-1-positive immunocompromised patients with and without PML.
人类多瘤病毒JC（JCV）可在艾滋病患者中引发人类进行性多灶性白质脑病（PML）。HIV-1阳性免疫功能正常和免疫功能低下患者的外周血单核细胞（PBMC）可携带JCV基因组，但其在PML发病前JCV潜伏或激活状态中的精确作用仍属假设。
评估无PML的HIV-1阳性免疫功能低下患者PBMC中JCV的潜伏或激活状态。
将一组82例无PML的HIV-1阳性免疫功能低下患者与10例患有AIDS和PML的患者以及69例无PML的HIV-1阳性免疫功能正常患者进行比较。
从PBMC中提取DNA和总RNA。通过半巢式聚合酶链反应（PCR）检测JCV DNA的存在。使用针对早期基因T和晚期基因VP1的引物对，可通过逆转录酶（RT）PCR鉴定PBMC中早期和晚期基因mRNA的表达。
在69例HIV-1阳性免疫功能正常患者中，17.4%通过PCR检测到JCV DNA；在82例HIV-1阳性免疫功能低下患者中，23.2%检测到JCV DNA；在10例患有AIDS和PML的患者中，60%检测到JCV DNA。PBMC中JCV DNA的检测与HIV-1阳性患者的临床或生物学状态之间无相关性。通过RT-PCR程序，在任何患者的PBMC中均未发现JCV早期和晚期mRNA的表达。
在151例HIV-1感染患者中，20.5%的患者PBMC中可检测到JCV DNA，这与疾病控制和预防中心（CDC）的感染阶段无关。此外，我们的结果表明，在有或无PML的HIV-1阳性免疫功能低下患者中，JCV在PBMC中的活跃复制似乎不存在或至少是非常罕见的事件。
