白花丹生物碱组分的免疫调节作用研究。

Studies on the immunomodulatory effects of Boerhaavia diffusa alkaloidal fraction.

作者信息

Mungantiwar A A, Nair A M, Shinde U A, Dikshit V J, Saraf M N, Thakur V S, Sainis K B

机构信息

Department of Pharmacology, The Bombay College of Pharmacy, Kalina, Mumbai, India.

出版信息

J Ethnopharmacol. 1999 May;65(2):125-31. doi: 10.1016/s0378-8741(98)00153-6.

DOI:10.1016/s0378-8741(98)00153-6

PMID:10465652

Abstract

The alkaloidal fraction of Boerhaavia diffusa was studied for its effect on cellular and humoral functions in mice. Oral administration of the fraction (25-100 mg/kg) significantly inhibited SRBC-induced delayed hypersensitivity reactions in mice. However, the inhibition was observed only during post-immunisation drug treatment, while no effect during pre-immunisation drug treatment was observed. A significant dose-related increase in antibody titre was observed during pre- and post-immunisation treatment. The alkaloidal fraction failed to show any blastogenic responsiveness of murine splenocytes to Concanvalin A (Con A) and lipopolysaccharide (LPS). Similarly, it did not display any mitogenic activity. Thus, the present study has shown the in vivo immunostimulatory activity of B. diffusa alkaloidal fraction without an in vitro effect.

摘要

对白花丹的生物碱部分进行了研究，以观察其对小鼠细胞和体液功能的影响。口服该部分（25 - 100毫克/千克）可显著抑制小鼠中由绵羊红细胞（SRBC）诱导的迟发型超敏反应。然而，仅在免疫后药物治疗期间观察到抑制作用，而在免疫前药物治疗期间未观察到任何效果。在免疫前和免疫后治疗期间均观察到抗体滴度有显著的剂量相关增加。该生物碱部分未能显示出小鼠脾细胞对刀豆球蛋白A（Con A）和脂多糖（LPS）的任何增殖反应性。同样，它也未表现出任何促有丝分裂活性。因此，本研究表明白花丹生物碱部分具有体内免疫刺激活性，但无体外效应。