Poulos L M, O'Meara T J, Sporik R, Tovey E R
Institute of Respiratory Medicine, University of Sydney, Sydney, Australia.
Clin Exp Allergy. 1999 Sep;29(9):1232-8. doi: 10.1046/j.1365-2222.1999.00613.x.
Measurement of personal exposure to Der p 1 aeroallergen has previously been limited by the low quantity of material collected by sampling systems and the assay sensitivity. This has meant that exposure could only be detected if long sampling periods were used or reservoir dust was artificially disturbed. We have developed a sampling method to sample true personal exposure and combined it with a novel method which is sensitive enough to measure allergen exposure over shorter time frames.
To describe normal domestic exposure to dust mite allergen during a range of activities in houses in Sydney, Australia.
Inhaled particles containing mite allergen Der p 1 were collected using a nasal air sampler which impacts particles (> approximately 5 microm) onto a protein-binding membrane coated with a thin, porous, adhesive film. The allergen is bound to the membrane in the immediate vicinity of the particle and detected by immunostaining with monoclonal antibodies specific for Der p 1. In addition, samples were collected using a standard Institute of Occupational Medicine (IOM) personal air sampler and the amount of eluted Der p 1 was assayed by ELISA.
The median number (range) of inhaled particles containing Der p 1 collected in each 10-min sampling period was: dust raising 5 (2-10); lying in bed, 0 (0-2); sitting on the bed, 1 (0-2); walking around the bedroom, 0 (0-2). This represented 0-5.1% of all particles captured. The Der p 1 concentration of floor and bed dust was 19.4 and 55.1 microg/g, respectively. The standard IOM personal sampler and ELISA were unable to detect Der p 1 for any of the activities performed.
We were able to count individual allergen-carrying particles inhaled over short time periods, during different domestic exposure situations. This will offer new insight into several aspects of personal allergen exposure.
以往，个人对屋尘螨变应原1（Der p 1）的暴露量测量受到采样系统收集的物质数量少以及检测灵敏度的限制。这意味着只有使用较长的采样时间或人为扰动储集灰尘，才能检测到暴露情况。我们开发了一种采样方法来采集真实的个人暴露样本，并将其与一种新方法相结合，该新方法灵敏度足以在更短的时间范围内测量变应原暴露量。
描述澳大利亚悉尼房屋内一系列活动期间家庭环境中对尘螨变应原的正常暴露情况。
使用鼻空气采样器收集含有螨变应原Der p 1的吸入颗粒，该采样器将颗粒（>约5微米）撞击到涂有薄的、多孔的、粘性膜的蛋白质结合膜上。变应原在颗粒紧邻处与膜结合，并通过用针对Der p 1的单克隆抗体进行免疫染色来检测。此外，使用标准的职业医学研究所（IOM）个人空气采样器收集样本，并通过酶联免疫吸附测定法（ELISA）测定洗脱的Der p 1量。
在每个10分钟采样期收集的含有Der p 1的吸入颗粒的中位数（范围）为：扬起灰尘时5（2 - 10）；躺在床上时，0（0 - 2）；坐在床上时，1（0 - 2）；在卧室走动时，0（0 - 2）。这占所有捕获颗粒的0 - 5.1%。地板和床上灰尘的Der p 1浓度分别为19.4和55.1微克/克。标准的IOM个人采样器和ELISA无法检测到所进行的任何活动中的Der p 1。
我们能够在不同的家庭暴露情况下，对短时间内吸入的携带变应原的单个颗粒进行计数。这将为个人变应原暴露的几个方面提供新的见解。
