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人铁蛋白的酶免疫测定法。

Enzyme immunoassay for human ferritin.

作者信息

Watanabe N, Niitsu Y, Ohtsuka S, Koseki J, Kohgo Y, Urushizaki I, Kato K, Ishikawa E

出版信息

Clin Chem. 1979 Jan;25(1):80-2.

PMID:104809
Abstract

We describe an enzyme immunoassay with use of beta-D-galactosidase for quantitation of ferritin in human serum. The minimum detectable ferritin concentration is 0.25 microgram/L of serum, which is comparable to results obtained by radioimmunoassay. The correlation coefficient between values determined by enzyme immunoassay and radioimmunoassay was 0.95 (n - 1 = 85, p less than 0.001).

摘要

我们描述了一种使用β-D-半乳糖苷酶的酶免疫测定法,用于定量人血清中的铁蛋白。可检测到的铁蛋白最低浓度为0.25微克/升血清,这与放射免疫测定法得到的结果相当。酶免疫测定法和放射免疫测定法所测值之间的相关系数为0.95(n - 1 = 85,p < 0.001)。

相似文献

1
Enzyme immunoassay for human ferritin.人铁蛋白的酶免疫测定法。
Clin Chem. 1979 Jan;25(1):80-2.
2
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Determination of ferritin in urine and in serum of normal adults with a sensitive enzyme immunoassay.用灵敏的酶免疫测定法测定正常成年人尿液和血清中的铁蛋白。
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A solid phase enzyme immunoassay for the quantitation of serum ferritin.一种用于定量血清铁蛋白的固相酶免疫测定法。
Clin Chim Acta. 1981 May 5;112(2):241-6. doi: 10.1016/0009-8981(81)90383-1.

引用本文的文献

1
Performance and comparability of laboratory methods for measuring ferritin concentrations in human serum or plasma: A systematic review and meta-analysis.用于测量人血清或血浆中铁蛋白浓度的实验室方法的性能和可比性:系统评价和荟萃分析。
PLoS One. 2018 May 3;13(5):e0196576. doi: 10.1371/journal.pone.0196576. eCollection 2018.
2
Latex agglutination test for ferritin measurement.用于铁蛋白测定的乳胶凝集试验。
J Clin Lab Anal. 2003;17(6):223-8. doi: 10.1002/jcla.10096.
3
Enzyme-linked immunosorbent assay (ELISA): a practical tool for rapid diagnosis of viruses and other infectious agents.
酶联免疫吸附测定(ELISA):一种用于快速诊断病毒及其他感染因子的实用工具。
Yale J Biol Med. 1980 Jan-Feb;53(1):85-92.
4
Enzyme immunoassays in which biotinillated beta-lactamase is used for the detection of microbial antigens.使用生物素化β-内酰胺酶检测微生物抗原的酶免疫测定法。
J Clin Microbiol. 1984 Mar;19(3):356-60. doi: 10.1128/jcm.19.3.356-360.1984.
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Rapid and accurate viral diagnosis.快速准确的病毒诊断。
Pharmacol Ther. 1989;40(2):287-328. doi: 10.1016/0163-7258(89)90098-3.