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白茅免疫刺激多糖的分离与部分特性研究

Isolation and partial characterization of immunostimulating polysaccharides from Imperata cylindrica.

作者信息

Pinilla V, Luu B

机构信息

Laboratoire de Chimie Organique des Substances Naturelles, UMR 7509-CNRS, Université Louis Pasteur, Strasbourg, France.

出版信息

Planta Med. 1999 Aug;65(6):549-52. doi: 10.1055/s-1999-14087.

DOI:10.1055/s-1999-14087
PMID:10483377
Abstract

The water-soluble crude extract prepared from Imperata cylindrica (Beauv.) was investigated for its immunomodulating activity. A set of polysaccharides with high molecular weights has been isolated by fractionation using gel filtration and anion-exchange chromatography. Each step of purification was monitored by bioassays. The presence of six monosaccharides has been established by chemical analysis. Quantitative analysis showed that the ratio of these monosaccharides differed from one polysaccharide to another. The crude extract as well as some of the purified polysaccharides enhance the proliferation of murine splenocytes.

摘要

对从白茅中制备的水溶性粗提物的免疫调节活性进行了研究。通过凝胶过滤和阴离子交换色谱分级分离，分离出了一组高分子量的多糖。纯化的每一步都通过生物测定进行监测。通过化学分析确定了六种单糖的存在。定量分析表明，这些单糖的比例因多糖不同而有所差异。粗提物以及一些纯化的多糖可增强小鼠脾细胞的增殖。

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