Zhang Y, Kiyohara H, Matsumoto T, Yamada H
Oriental Medicine Research Center, Kitasato Institute, Tokyo, Japan.
Planta Med. 1997 Oct;63(5):393-9. doi: 10.1055/s-2006-957720.
A crude polysaccharide fraction (DAP-1) was prepared from roots of Dipsacus asperoides by hot water extraction and EtOH precipitation, and tested for anti-complementary activity, mitogenic activity of lymphocytes, and effects on acid phosphatase and phagocytic activities of macrophages. DAP-1 showed not only anti-complementary activity but also a stimulating effect on the mitogenic activity of lymphocytes. DAP-1 also significantly suppressed the phagocytic activity of macrophages. Although DAP-1 directly stimulated the mitogenecity of lymphocytes, it had no effect on lipopolysaccharide- or concanavalin A-induced mitogenic activity of lymphocytes. Periodate oxidation and pronase digestion suggested that the polysaccharide moiety in DAP-1 contributed to the expression of its anti-complementary and mitogenic activities and that the protein moiety in DAP-1 was responsible for its effect on phagocytosis. DAP-1 gave three polysaccharide fractions (DAP-2, 3, and 4) by fractionation using cetyltrimethylammonium bromide. All the fractions had potent anti-complementary activity, but showed different stimulating or suppressive effects on the proliferation of lymphocytes, phagocytosis, or acid phosphatase activity. Three potent anti-complementary polysaccharides (DAP-4I-1a, DAP-4I-1b, and DAP-4IIa-1) were purified from DAP-4 by anion-exchange chromatography, gel filtration, and HPLC. DAP-4I-1a, I-1b, and IIa-1 consisted of Ara, Rha, Xyl, Gal, Glc and GlcA in a molar ratio of 1.0:0.7:1.0:18.6:22.2:nil; 1.0:0.1:0.3:19.3:26.8:nil; and 3.7:trace:0.6:26.3:5.5:1.0; respectively. Among the polysaccharides, only DAP-4IIa-1 reacted with beta-glucosyl-Yariv antigen. Methylation analysis indicated that DAP-4I-1a mainly comprised 4-linked Gal and 3-, 4-, and 6-linked Glc, whereas DAP-4IIa-1 consisted mainly of terminal Araf, 3-linked Glc, and 3,6-branched Gal.
通过热水提取和乙醇沉淀从川续断根中制备了一种粗多糖组分（DAP-1），并对其进行了抗补体活性、淋巴细胞促有丝分裂活性以及对巨噬细胞酸性磷酸酶和吞噬活性影响的测试。DAP-1不仅具有抗补体活性，还对淋巴细胞的促有丝分裂活性有刺激作用。DAP-1还显著抑制巨噬细胞的吞噬活性。尽管DAP-1直接刺激淋巴细胞的有丝分裂原性，但对脂多糖或伴刀豆球蛋白A诱导的淋巴细胞促有丝分裂活性没有影响。高碘酸盐氧化和链霉蛋白酶消化表明，DAP-1中的多糖部分有助于其抗补体和促有丝分裂活性的表达，而DAP-1中的蛋白质部分则对其吞噬作用负责。通过使用十六烷基三甲基溴化铵分级分离，DAP-1得到了三个多糖组分（DAP-2、3和4）。所有组分都具有很强的抗补体活性，但对淋巴细胞增殖、吞噬作用或酸性磷酸酶活性表现出不同的刺激或抑制作用。通过阴离子交换色谱、凝胶过滤和高效液相色谱从DAP-4中纯化出三种有效的抗补体多糖（DAP-4I-1a、DAP-4I-1b和DAP-4IIa-1）。DAP-4I-1a、I-1b和IIa-1分别由阿拉伯糖、鼠李糖、木糖、半乳糖、葡萄糖和葡萄糖醛酸以1.0:0.7:1.0:18.6:22.2:无；1.0:0.1:0.3:19.3:26.8:无；和3.7:痕量:0.6:26.3:5.5:1.0的摩尔比组成。在这些多糖中，只有DAP-4IIa-1与β-葡萄糖基-Yariv抗原发生反应。甲基化分析表明，DAP-4I-1a主要由4-连接的半乳糖和3-、4-和6-连接的葡萄糖组成，而DAP-4IIa-1主要由末端阿拉伯糖、3-连接的葡萄糖和3,6-分支的半乳糖组成。
