Marra K G, Szem J W, Kumta P N, DiMilla P A, Weiss L E
The Institute for Complex Engineered Systems, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA.
J Biomed Mater Res. 1999 Dec 5;47(3):324-35. doi: 10.1002/(sici)1097-4636(19991205)47:3<324::aid-jbm6>3.0.co;2-y.
Blends of biodegradable polymers, poly(caprolactone) and poly(D, L-lactic-co-glycolic acid), have been examined as scaffolds for applications in bone tissue engineering. Hydroxyapatite granules have been incorporated into the blends and porous discs were prepared. Mechanical properties and degradation rates in vitro of the composites were determined. The discs were seeded with rabbit bone marrow or cultured bone marrow stromal cells and incubated under physiological conditions. Polymer/ceramic scaffolds supported cell growth throughout the scaffold for 8 weeks. Scanning and transmission electron microscopy, and histological analyses were used to characterize the seeded composites. This study suggests the feasibility of using novel polymer/ceramic composites as scaffold in bone tissue engineering applications.
已对可生物降解聚合物聚己内酯和聚（D，L-乳酸-乙醇酸共聚物）的共混物作为骨组织工程应用支架进行了研究。将羟基磷灰石颗粒加入到共混物中并制备了多孔圆盘。测定了复合材料的力学性能和体外降解速率。将圆盘接种兔骨髓或培养的骨髓基质细胞，并在生理条件下孵育。聚合物/陶瓷支架在整个支架上支持细胞生长达8周。使用扫描电子显微镜、透射电子显微镜和组织学分析对接种细胞的复合材料进行表征。本研究表明在骨组织工程应用中使用新型聚合物/陶瓷复合材料作为支架的可行性。
