Barile G R, Chang S S, Park L S, Reppucci V S, Schiff W M, Schmidt A M
The Edward S Harkness Eye Institute St Luke's-Roosevelt Hospital Center Columbia University College of Physicians & Surgeons Department of Ophthalmology NY, 10032, USA.
Curr Eye Res. 1999 Sep;19(3):219-27. doi: 10.1076/ceyr.19.3.219.5314.
To measure vitreous levels of soluble intercellular adhesion molecule-1 (sICAM-1) and soluble vascular cellular adhesion molecule-1 (sVCAM-1) in the eyes of patients with retinal detachment (RD) due to proliferative diabetic retinopathy (PDR) or proliferative vitreoretinopathy (PVR) and to determine whether the levels of these mediators correlated with clinical parameters of disease.
Undiluted vitreous specimens were collected from 50 eyes of 48 patients undergoing vitrectomy for traction RD due to PDR (21 specimens) and recurrent RD due to PVR (19 specimens). Control vitreous specimens were obtained from patients undergoing macular hole repair (10 specimens). The levels of sICAM-1 and sVCAM-1 were measured in each sample by specific enzyme-linked immunoadsorbent assays.
Vitreous levels of sICAM-1 were significantly increased in vitreous specimens from both PVR (median +/- SD; 12.0 +/- 76.3 ng/ml; P < 0.01) and PDR (8.4 +/- 24.0 ng/ml; P < 0.01) when compared to vitreous from eyes with macular holes (0. 3 +/- 4.2 ng/ml). Vitreous levels of sVCAM-1 were significantly increased in both PVR (36.5 +/- 255.2 ng/ml; P < 0.001) and PDR (26. 2 +/- 93.5 ng/ml; P < 0.01) when compared to control vitreous (17.7 +/- 7.8 ng/ml). The vitreous levels of sICAM-1 were higher in cases of PDR which developed recurrent proliferative disease (P < 0.01) and recurrent RD (P = 0.01), whereas the levels of sICAM-1 in PVR and sVCAM-1 in PDR and PVR did not significantly correlate with these clinical parameters.
Soluble forms of ICAM-1 and VCAM-1 are increased in the vitreous cavity of patients with RD due to PDR or PVR, reflecting the inflammatory nature of these conditions and suggesting a possible role for these mediators in the pathogenesis of proliferative retinal disease. The vitreous levels of these sCAMs at the time of surgery may serve as a marker of inflammation, but their specific levels do not predict the likelihood of recurrent proliferation or surgical anatomic success in most cases of PVR and PDR.
检测因增殖性糖尿病视网膜病变（PDR）或增殖性玻璃体视网膜病变（PVR）导致视网膜脱离（RD）患者眼内可溶性细胞间黏附分子 -1（sICAM -1）和可溶性血管细胞黏附分子 -1（sVCAM -1）的玻璃体水平，并确定这些介质水平是否与疾病的临床参数相关。
从48例因PDR导致牵引性RD（21份标本）和因PVR导致复发性RD（19份标本）而接受玻璃体切除术的患者的50只眼中收集未稀释的玻璃体标本。对照玻璃体标本取自接受黄斑裂孔修复术的患者（10份标本）。通过特异性酶联免疫吸附测定法测量每个样本中sICAM -1和sVCAM -1的水平。
与黄斑裂孔眼的玻璃体（0.3±4.2 ng/ml）相比，PVR（中位数±标准差；12.0±76.3 ng/ml；P<0.01）和PDR（8.4±24.0 ng/ml；P<0.01）患者的玻璃体标本中sICAM -1的玻璃体水平显著升高。与对照玻璃体（17.7±7.8 ng/ml）相比，PVR（36.5±255.2 ng/ml；P<0.001）和PDR（26.2±93.5 ng/ml；P<0.01）患者的玻璃体中sVCAM -1的玻璃体水平均显著升高。在发生复发性增殖性疾病（P<0.01）和复发性RD（P = 0.01）的PDR病例中，sICAM -1的玻璃体水平较高，而PVR中的sICAM -1水平以及PDR和PVR中的sVCAM -1水平与这些临床参数无显著相关性。
因PDR或PVR导致RD的患者玻璃体腔中ICAM -1和VCAM -1的可溶性形式增加，反映了这些疾病的炎症性质，并提示这些介质在增殖性视网膜疾病发病机制中可能发挥作用。手术时这些sCAMs的玻璃体水平可作为炎症标志物，但在大多数PVR和PDR病例中，其具体水平并不能预测复发增殖的可能性或手术解剖成功的可能性。
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