El-Asrar Ahmed M Abu, Nawaz Mohd Imtiaz, Kangave Dustan, Geboes Karel, Ola Mohammad Shamsul, Ahmad Saif, Al-Shabrawey Mohamed
Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
Mol Vis. 2011;17:1829-38. Epub 2011 Jul 6.
To measure levels of high-mobility group box -1 (HMGB1) and soluble receptor for advanced glycation end products (sRAGE) in the vitreous fluid from patients with proliferative diabetic retinopathy (PDR) and to correlate their levels with clinical disease activity and the levels of the inflammatory biomarkers monocyte chemoattractant protein-1 (MCP-1), soluble intercellular adhesion molecule-1 (sICAM-1), interleukin-1β (IL-1β), and granulocyte macrophage colony-stimulating factor (GM-CSF). In addition, we examined the expression of HMGB1 in the retinas of diabetic mice.
Vitreous samples from 29 PDR and 17 nondiabetic patients were studied by enzyme-linked immunosorbent assay. Retinas of mice were examined by immunofluorescence analysis and western blotting.
HMGB1 was detected in all vitreous samples and sRAGE was detected in 5 PDR samples. IL-1β was detected in 3PDR samples and GM-CSF was not detected. Mean HMGB1 levels in PDR with active neovascularization were twofold and threefold higher than that in inactive PDR and nondiabetic patients, respectively. Mean HMGB1 levels in PDR patients with hemorrhage were significantly higher than those in PDR patients without hemorrhage and nondiabetic patients (p=0.0111). There were significant correlations between levels of HMGB1 and levels of MCP-1 (r=0.333, p=0.025) and sICAM-1 (r=0.548, p<0.001). HMGB1 expression was also upregulated in the retinas of diabetic mice.
Subclinical chronic inflammation might contribute to the progression of PDR.
检测增殖性糖尿病视网膜病变（PDR）患者玻璃体液中高迁移率族蛋白B1（HMGB1）和晚期糖基化终末产物可溶性受体（sRAGE）水平，并将其与临床疾病活动度以及炎症生物标志物单核细胞趋化蛋白-1（MCP-1）、可溶性细胞间黏附分子-1（sICAM-1）、白细胞介素-1β（IL-1β）和粒细胞巨噬细胞集落刺激因子（GM-CSF）水平进行关联分析。此外，我们还检测了糖尿病小鼠视网膜中HMGB1的表达情况。
采用酶联免疫吸附测定法研究29例PDR患者和17例非糖尿病患者的玻璃体液样本。通过免疫荧光分析和蛋白质印迹法检测小鼠视网膜。
所有玻璃体液样本中均检测到HMGB1，5例PDR样本中检测到sRAGE。3例PDR样本中检测到IL-1β，未检测到GM-CSF。有活动性新生血管形成的PDR患者的平均HMGB1水平分别是非活动性PDR患者和非糖尿病患者的两倍和三倍。有出血的PDR患者的平均HMGB1水平显著高于无出血的PDR患者和非糖尿病患者（p = 0.0111）。HMGB1水平与MCP-1水平（r = 0.333，p = 0.025）和sICAM-1水平（r = 0.548，p < 0.001）之间存在显著相关性。糖尿病小鼠视网膜中HMGB1表达也上调。
亚临床慢性炎症可能促进PDR的进展。
