Prieto G, Simón F, Genchi C, McCall J W, Venco L
Laboratorio de Parasitologia, Facultad de Farmacia, Salamanca, Spain.
Vet Parasitol. 1999 Sep 15;86(1):5-13. doi: 10.1016/s0304-4017(99)00116-8.
Early detection of feline dirofilariosis was achieved by ELISA testing using adult somatic (SA) and excretory/secretory (E/S) antigens. Sera from experimentally infected cats showed significantly higher IgG levels at 2 months post-infection (p.i.) when compared to sera obtained prior to infection. This difference was apparent at all serum dilutions (1:100 to 1:800) in ELISA(SA) and at all dilutions except 1:800 when tested with ELISA(E/S). Changes in specific anti-SA and anti-E/S IgG levels were found in infected cats treated with the macrolid parasiticide. ELISA(E/S) was able to detect a significant decrease in IgG levels in ivermectin treated cats at 3 months post-treatment (p.t.), compared to infected, untreated controls. However, testing with ELISA(SA) showed a significant decrease in antibody levels in treated cats only at 4 months after treatment. Sera from untreated cats recognized previously identified specific markers for feline dirofilariosis on SA (22, 26, 30 and 40 kDa) and on E/S (22 and 25 kDa) in Western blot analysis. Sera from treated cats, on the other hand, gave only weak bands or none at all, indicating a decrease in antibody concentration. These results suggest that serologic testing with ELISA is useful in the early detection of Dirofilaria immitis infection and, together with Western blot analysis, can assist in the evaluation of response to chemoprophylactic treatment of feline dirofilariosis, two aspects of prime importance in the prevention of this disease in cats.
通过使用成虫体细胞（SA）抗原和排泄/分泌（E/S）抗原的ELISA检测实现了猫心丝虫病的早期检测。与感染前获得的血清相比，实验感染猫的血清在感染后2个月（p.i.）时显示出显著更高的IgG水平。在ELISA（SA）中，所有血清稀释度（1:100至1:800）下这种差异都很明显；在用ELISA（E/S）检测时，除1:800外的所有稀释度下差异也很明显。在用大环内酯类驱虫剂治疗的感染猫中发现了特异性抗SA和抗E/S IgG水平的变化。与未治疗的感染对照相比，ELISA（E/S）能够检测到伊维菌素治疗的猫在治疗后3个月（p.t.）时IgG水平显著下降。然而，用ELISA（SA）检测显示，治疗后的猫仅在治疗后4个月时抗体水平显著下降。在蛋白质印迹分析中，未治疗猫的血清识别出先前确定的猫心丝虫病的特异性标志物，SA上的（22、26、30和40 kDa）以及E/S上的（22和25 kDa）。另一方面，治疗后猫的血清仅产生弱条带或根本没有条带，表明抗体浓度降低。这些结果表明，ELISA血清学检测在犬恶丝虫感染的早期检测中很有用，并且与蛋白质印迹分析一起，可以协助评估猫心丝虫病化学预防治疗的反应，这是预防猫这种疾病的两个至关重要的方面。
