Santamaría B, Cordero M, Muro A, Simón F
Laboratorio de Parasitología, Facultad de Farmacia, Salamanca, Spain.
Parasite. 1995 Sep;2(3):269-73. doi: 10.1051/parasite/1995023269.
The usefulness of excretory/secretory (E/S) products of adult Dirofilaria immitis for the diagnosis of human dirofilariosis was evaluated in 97 human sera using an ELISA and a Western Blot analysis with E/S antigens. Results of the ELISA analysis show that in healthy individuals from an endemic area seropositivity is high (22%). Immuno Blot analysis shows that the reaction pattern with E/S antigens differentiates healthy seropositives, which recognize a 43 kDa band, from patients with pulmonary dirofilariosis, which specifically recognize bands between 22-28 kDa.
利用酶联免疫吸附测定（ELISA）和用排泄/分泌（E/S）抗原进行的蛋白质印迹分析，在97份人血清中评估了成年犬恶丝虫的排泄/分泌（E/S）产物对人类恶丝虫病诊断的效用。ELISA分析结果表明，在来自流行地区的健康个体中，血清阳性率很高（22%）。免疫印迹分析表明，E/S抗原的反应模式可区分健康血清阳性者（识别一条43 kDa的条带）和肺恶丝虫病患者（特异性识别22 - 28 kDa之间的条带）。
