Becher P, Shannon A D, Tautz N, Thiel H J
Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
Virology. 1994 Feb;198(2):542-51. doi: 10.1006/viro.1994.1065.
Three serologically different pestivirus strains isolated from sheep were selected for molecular analysis. cDNA and deduced amino acid sequences of the genomic regions encoding glycoproteins E1 and E2 were obtained from the three strains. A comparison with amino acid sequences of bovine viral diarrhea virus (BVDV) and classical swine fever virus (CSFV) revealed that one of the three ovine pestivirus strains can be grouped together with BVDV. The other two strains, however, were clearly different from both BVDV and CSFV. Surprisingly, the amino acid sequences from these two viruses were more similar to CSFV than to BVDV. The identity between so-called "true" BDV strains at the amino acid level is about 95% for E1 and 86% for E2 and thus similar to homologies found between CSFV strains. For one "true" BDV strain the genomic region encompassing the nonstructural protein p125 was also cloned and sequenced. The respective comparative analysis led to results which are similar to the ones obtained for the two structural glycoproteins. Taken together the data demonstrate that "true" border disease virus strains represent a separate group within the genus pestivirus.
从绵羊中分离出的三种血清学不同的瘟病毒株被选用于分子分析。获得了这三种毒株编码糖蛋白E1和E2的基因组区域的cDNA及推导的氨基酸序列。与牛病毒性腹泻病毒（BVDV）和经典猪瘟病毒（CSFV）的氨基酸序列比较显示，三种绵羊瘟病毒株中的一种可与BVDV归为一组。然而，另外两种毒株与BVDV和CSFV均明显不同。令人惊讶的是，这两种病毒的氨基酸序列与CSFV的相似性高于与BVDV的相似性。所谓“真正的”BDV毒株在氨基酸水平上，E1的同一性约为95%，E2的同一性约为86%，因此与CSFV毒株之间的同源性相似。对于一种“真正的”BDV毒株，还克隆并测序了包含非结构蛋白p125的基因组区域。各自的比较分析得出的结果与两种结构糖蛋白的结果相似。综合这些数据表明，“真正的”边界病病毒株在瘟病毒属内代表一个独立的组。
