Donohoe M E, Zhang X, McGinnis L, Biggers J, Li E, Shi Y
Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Mol Cell Biol. 1999 Oct;19(10):7237-44. doi: 10.1128/MCB.19.10.7237.
Yin Yang 1 (YY1) is a zinc finger-containing transcription factor and a target of viral oncoproteins. To determine the biological role of YY1 in mammalian development, we generated mice deficient for YY1 by gene targeting. Homozygosity for the mutated YY1 allele results in embryonic lethality in the mouse. YY1 mutants undergo implantation and induce uterine decidualization but rapidly degenerate around the time of implantation. A subset of YY1 heterozygote embryos are developmentally retarded and exhibit neurulation defects, suggesting that YY1 may have additional roles during later stages of mouse embryogenesis. Our studies demonstrate an essential function for YY1 in the development of the mouse embryo.
阴阳1（YY1）是一种含锌指结构的转录因子，也是病毒癌蛋白的作用靶点。为了确定YY1在哺乳动物发育中的生物学作用，我们通过基因打靶技术构建了YY1基因缺陷小鼠。YY1等位基因突变的纯合子会导致小鼠胚胎致死。YY1突变体能够着床并诱导子宫蜕膜化，但在着床前后会迅速退化。一部分YY1杂合子胚胎发育迟缓并表现出神经管形成缺陷，这表明YY1在小鼠胚胎发育后期可能具有其他作用。我们的研究证明了YY1在小鼠胚胎发育过程中具有重要功能。
