Berktas Mustafa, Aydin Sabahattin, Yilmaz Yuksel, Cecen Kursat, Bozkurt Hamza
Microbiology, Yuzuncü Yil University Medical Faculty, Cumhuriyet Cad., 65200 Van, Turkey.
Int Urol Nephrol. 2008;40(2):383-9. doi: 10.1007/s11255-007-9289-4.
We investigated the relationship between semen motility and various uropathogenic microorganisms.
Semen specimens from healthy donors were divided into portions and incubated with uropathogenic microorganisms in concentrations varying from 2 x 10 to 2 x 10(7) microorganisms/ml(-1). Uninfected suspensions of spermatozoa served as controls. In all samples, sperm motility was examined at the second, fourth, and sixth hours after incubation in order to assess motility as a function of time. Enterobacter aerogenes, Staphylococcus epidermidis, S. aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli were used as bacterial agents. Besides these bacterial strains, Candida albicans was also used.
Observed negative impact on sperm motility was not correlated with microorganism concentration. However, until a certain concentration threshold, this impact was prominent. Regardless of the microorganism, this deleterious effect could not be confirmed in specimens coincubated with lower microorganism concentration. No or poor correlation was found between motility and bacteria concentration except with E. aerogenes at the second hour. The data indicates that sperm function impairment is not related to direct sperm and bacteria interaction. Instead, bacterial concentration enough to change the environment or to consume high energy might result in motility loss.
我们研究了精液活力与各种尿路致病性微生物之间的关系。
将健康供体的精液标本分成多份，与浓度从2×10至2×10⁷个微生物/毫升的尿路致病性微生物一起孵育。未感染的精子悬浮液作为对照。在所有样本中，孵育后第二、第四和第六小时检查精子活力，以评估活力随时间的变化。产气肠杆菌、表皮葡萄球菌、金黄色葡萄球菌、铜绿假单胞菌、肺炎克雷伯菌和大肠杆菌用作细菌制剂。除了这些细菌菌株外，白色念珠菌也被使用。
观察到的对精子活力的负面影响与微生物浓度无关。然而，在达到一定浓度阈值之前，这种影响是显著的。无论微生物种类如何，在与较低微生物浓度共同孵育的标本中均未证实这种有害作用。除了在第二小时与产气肠杆菌外，活力与细菌浓度之间未发现或仅有微弱的相关性。数据表明精子功能损害与精子和细菌的直接相互作用无关。相反，足以改变环境或消耗大量能量的细菌浓度可能导致活力丧失。
