Division of Urology, Department of Surgery, University of Toronto, Ontario, Canada.
Fertil Steril. 2012 May;97(5):1050-5. doi: 10.1016/j.fertnstert.2012.01.124. Epub 2012 Feb 16.
To determine the incidence of bacteriospermia and elevated seminal leukocytes (ESL) in a subfertile male population and correlate these results with semen parameters.
Retrospective cohort study.
Canadian tertiary-level male infertility clinic and university-affiliated andrology and microbiology laboratories.
PATIENT(S): Four thousand nine hundred thirty-five nonazoospermic subfertile men.
INTERVENTION(S): Analysis and concurrent culture of 7,852 semen samples.
MAIN OUTCOME MEASURE(S): Incidence of bacteriospermia and ESL and comparison of semen parameters between these groups.
RESULT(S): The rate of bacteriospermia was 15% (22 species), and the rate of ESL was 19%, with no statistical correlation between these groups. Bacteriospermic patients (without ESL) had a statistically significant deterioration in DNA fragmentation index (DFI) only, compared with patients without bacteriospermia and ESL (24.1 vs. 21.8%). ESL alone was associated with a statistically significant deterioration in sperm concentration (20.6 vs. 55.3 × 10(6)/mL), motility (21.8 vs. 26.9%), normal morphology (12.3 vs. 17.4%), and DFI (26.5 vs. 21.8%), with no additional deterioration identified with bacteriospermia.
CONCLUSION(S): Bacteriospermia and ESL were prevalent, but not statistically associated, in subfertile men. Bacteriospermia alone was associated with an increase in DFI only, but the presence of ESL was the dominant factor associated with deterioration in semen parameters.
确定患有不育症的男性人群中细菌精子症和精液白细胞增多症（ESL）的发生率，并将这些结果与精液参数相关联。
回顾性队列研究。
加拿大三级男性不育诊所和大学附属男科和微生物学实验室。
4935 名非无精子症的不育男性。
对 7852 份精液样本进行分析和并行培养。
细菌精子症和 ESL 的发生率以及这些组之间的精液参数比较。
细菌精子症的发生率为 15%（22 种），ESL 的发生率为 19%，两组之间无统计学相关性。与无细菌精子症和 ESL 的患者相比，细菌精子症患者（无 ESL）的 DNA 碎片指数（DFI）仅具有统计学显著恶化，分别为 24.1%和 21.8%。仅 ESL 与精子浓度（20.6 对 55.3×10⁶/ml）、活力（21.8 对 26.9%）、正常形态（12.3 对 17.4%）和 DFI（26.5 对 21.8%）的统计学显著恶化相关，与细菌精子症无进一步恶化。
细菌精子症和 ESL 在不育男性中普遍存在，但无统计学关联。单独的细菌精子症仅与 DFI 增加相关，但 ESL 的存在是与精液参数恶化相关的主要因素。
