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本文引用的文献

1
Quinolone resistance mutations in the GrlB protein of Staphylococcus aureus.金黄色葡萄球菌GrlB蛋白中的喹诺酮耐药性突变。
Antimicrob Agents Chemother. 1998 Nov;42(11):3044-6. doi: 10.1128/AAC.42.11.3044.
2
Active efflux of norfloxacin by Bacteroides fragilis.脆弱拟杆菌对诺氟沙星的主动外排
Antimicrob Agents Chemother. 1998 Aug;42(8):2119-21. doi: 10.1128/AAC.42.8.2119.
3
Inhibitory activities of quinolones against DNA gyrase and topoisomerase IV purified from Staphylococcus aureus.喹诺酮类药物对从金黄色葡萄球菌中纯化的DNA回旋酶和拓扑异构酶IV的抑制活性。
Antimicrob Agents Chemother. 1997 Nov;41(11):2362-6. doi: 10.1128/AAC.41.11.2362.
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Antimicrobial resistance in anaerobes.厌氧菌中的抗菌药物耐药性。
Clin Infect Dis. 1997 Jan;24 Suppl 1:S110-20. doi: 10.1093/clinids/24.supplement_1.s110.
5
Quinolone resistance locus nfxD of Escherichia coli is a mutant allele of the parE gene encoding a subunit of topoisomerase IV.大肠杆菌喹诺酮耐药位点nfxD是编码拓扑异构酶IV一个亚基的parE基因的突变等位基因。
Antimicrob Agents Chemother. 1997 Jan;41(1):175-9. doi: 10.1128/AAC.41.1.175.
6
In vitro susceptibility of anaerobes to quinolones in the United States.美国厌氧菌对喹诺酮类药物的体外敏感性
Clin Infect Dis. 1996 Dec;23 Suppl 1:S2-8. doi: 10.1093/clinids/23.supplement_1.s2.
7
Involvement of topoisomerase IV and DNA gyrase as ciprofloxacin targets in Streptococcus pneumoniae.拓扑异构酶IV和DNA促旋酶作为环丙沙星在肺炎链球菌中的作用靶点。
Antimicrob Agents Chemother. 1996 Oct;40(10):2321-6. doi: 10.1128/AAC.40.10.2321.
8
Quinolone-resistant mutants of escherichia coli DNA topoisomerase IV parC gene.大肠杆菌DNA拓扑异构酶IV parC基因的喹诺酮抗性突变体
Antimicrob Agents Chemother. 1996 Mar;40(3):710-14. doi: 10.1128/AAC.40.3.710.
9
Multidrug efflux pumps of gram-negative bacteria.革兰氏阴性菌的多药外排泵
J Bacteriol. 1996 Oct;178(20):5853-9. doi: 10.1128/jb.178.20.5853-5859.1996.
10
Cloning and characterization of the parC and parE genes of Streptococcus pneumoniae encoding DNA topoisomerase IV: role in fluoroquinolone resistance.肺炎链球菌编码DNA拓扑异构酶IV的parC和parE基因的克隆与特性分析：在氟喹诺酮耐药性中的作用
J Bacteriol. 1996 Jul;178(14):4060-9. doi: 10.1128/jb.178.14.4060-4069.1996.

脆弱拟杆菌编码DNA促旋酶的gyrA和gyrB基因的分子克隆。

Molecular cloning of the gyrA and gyrB genes of Bacteroides fragilis encoding DNA gyrase.

作者信息

Onodera Y, Sato K

机构信息

New Product Research Laboratories I, Daiichi Pharmaceutical Co., Ltd., Edogawa-ku, Tokyo 134-8630, Japan.

出版信息

Antimicrob Agents Chemother. 1999 Oct;43(10):2423-9. doi: 10.1128/AAC.43.10.2423.

DOI:10.1128/AAC.43.10.2423
PMID:10508019
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC89495/
Abstract

The genes encoding the DNA gyrase A and B subunits of Bacteroides fragilis were cloned and sequenced. The gyrA and gyrB genes code for proteins of 845 and 653 amino acids, respectively. These proteins were expressed in Escherichia coli, and the combination of GyrA and GyrB exhibited ATP-dependent supercoiling activity. To analyze the role of DNA gyrase in quinolone resistance of B. fragilis, we isolated mutant strains by stepwise selection for resistance to increasing concentrations of levofloxacin. We analyzed the resistant mutants and showed that Ser-82 of GyrA, equivalent to resistance hot spot Ser-83 of GyrA in E. coli, was in each case replaced with Phe. These results suggest that DNA gyrase is an important target for quinolones in B. fragilis.

摘要

对脆弱拟杆菌的DNA促旋酶A和B亚基的编码基因进行了克隆和测序。gyrA和gyrB基因分别编码含845和653个氨基酸的蛋白质。这些蛋白质在大肠杆菌中表达，GyrA和GyrB的组合表现出ATP依赖性超螺旋活性。为了分析DNA促旋酶在脆弱拟杆菌对喹诺酮类耐药性中的作用，我们通过逐步选择对浓度递增的左氧氟沙星耐药的菌株来分离突变株。我们对耐药突变株进行了分析，结果表明，GyrA的第82位丝氨酸（相当于大肠杆菌中GyrA的耐药热点第83位丝氨酸）在每种情况下都被苯丙氨酸取代。这些结果表明，DNA促旋酶是喹诺酮类药物作用于脆弱拟杆菌的一个重要靶点。