Appl Environ Microbiol. 1997 Sep;63(9):3607-13. doi: 10.1128/aem.63.9.3607-3613.1997.
Chloroform (CF) degradation by a butane-grown enrichment culture, CF8, was compared to that by butane-grown Pseudomonas butanovora and Mycobacterium vaccae JOB5 and to that by a known CF degrader, Methylosinus trichosporium OB3b. All three butane-grown bacteria were able to degrade CF at rates comparable to that of M. trichosporium. CF degradation by all four bacteria required O(inf2). Butane inhibited CF degradation by the butane-grown bacteria, suggesting that butane monooxygenase is responsible for CF degradation. P. butanovora required exogenous reductant to degrade CF, while CF8 and M. vaccae utilized endogenous reductants. Prolonged incubation with CF resulted in decreased CF degradation. CF8 and P. butanovora were more sensitive to CF than either M. trichosporium or M. vaccae. CF degradation by all three butane-grown bacteria was inactivated by acetylene, which is a mechanism-based inhibitor for several monooxygenases. Butane protected all three butane-grown bacteria from inactivation by acetylene, which indicates that the same monooxygenase is responsible for both CF and butane oxidation. CF8 and P. butanovora were able to degrade other chlorinated hydrocarbons, including trichloroethylene, 1,2-cis-dichloroethylene, and vinyl chloride. In addition, CF8 degraded 1,1,2-trichloroethane. The results indicate the potential of butane-grown bacteria for chlorinated hydrocarbon transformation.
氯仿（CF）的降解由丁烷生长的富集培养物 CF8 进行，与丁烷生长的假单胞菌和结核分枝杆菌 JOB5 进行了比较，并与已知的 CF 降解菌 Methylosinus trichosporium OB3b 进行了比较。这三种丁烷生长的细菌都能够以与 M. trichosporium 相当的速率降解 CF。所有四种细菌降解 CF 都需要 O(inf2)。丁烷抑制了丁烷生长的细菌对 CF 的降解，表明丁烷单加氧酶是 CF 降解的原因。P. butanovora 需要外源还原剂来降解 CF，而 CF8 和 M. vaccae 则利用内源性还原剂。长时间接触 CF 会导致 CF 降解减少。CF8 和 P. butanovora 对 CF 的敏感性比 M. trichosporium 或 M. vaccae 都高。所有三种丁烷生长的细菌的 CF 降解都被乙炔失活，乙炔是几种单加氧酶的机制抑制剂。乙炔保护了所有三种丁烷生长的细菌免受失活，这表明相同的单加氧酶负责 CF 和丁烷的氧化。CF8 和 P. butanovora 能够降解其他氯化烃，包括三氯乙烯、1,2-顺式-二氯乙烯和氯乙烯。此外，CF8 还降解了 1,1,2-三氯乙烷。结果表明，丁烷生长的细菌在氯化烃转化方面具有潜力。
