Holdsworth S R, Glasgow E F, Atkins R C, Thomson N M
Nephron. 1978;22(4-6):454-9. doi: 10.1159/000181513.
A modified sieving technique has been developed to isolate pure glomeruli from monkey, sheep, dog, rabbit and rat kidney. Glomeruli from all these species have been grown in tissue culture and the glomerular cell outgrowth studied by light microscopy and time-lapse cinemicroscopy. The pattern of growth was the same for all the species studied. In all species, three cell populations have been identified with the features of epithelial cells, mesangial cells and macrophages, although the latter population is only rarely observed. The morphology and culture characteristics of each cell type in all species were similar, including the relative numbers present and rates of division.
一种改良的筛分技术已被开发出来，用于从猴、羊、狗、兔和大鼠的肾脏中分离出纯净的肾小球。来自所有这些物种的肾小球已在组织培养中生长，并通过光学显微镜和延时电影显微镜研究了肾小球细胞的生长情况。所有研究物种的生长模式都是相同的。在所有物种中，已识别出具有上皮细胞、系膜细胞和巨噬细胞特征的三种细胞群，尽管后者群体很少被观察到。所有物种中每种细胞类型的形态和培养特征都相似，包括细胞的相对数量和分裂速度。
