以微秒衰减时间铼金属-配体络合物作为供体时，扩散对溶液中能量转移的影响。
Effects of diffusion on energy transfer in solution using a microsecond decay time rhenium metal-ligand complex as the donor.
作者信息
Kuśba Józef, Piszczek Grzegorz, Gryczynski Ignacy, Johnson Michael L, Lakowicz Joseph R
机构信息
Faculty of Applied Physics and Mathematics, Technical University of Gdansk, Gdansk, Poland.
University of Maryland School of Medicine, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA.
出版信息
Chem Phys Lett. 2000 Mar;319(5-6):661-668. doi: 10.1016/s0009-2614(00)00071-3. Epub 2000 Mar 30.
Abstract
We used resonance energy transfer and frequency-domain fluorometry to measure slow donor to acceptor diffusion in viscous media. The frequency-domain RET data were analyzed using a new numerical algorithm for predicting the donor intensity decay in the presence of diffusion occurring within the donor decay time. By the use of a rhenium metal-ligand complex as a microsecond decay time donor we were able to measure mutual donor-to-acceptor diffusion coefficients as low as 2 × 10 cm/s. The availability of microsecond decay time luminophores and appropriate theory suggests the use of diffusion-enhanced energy transfer for measurement of diffusive processes and structural dynamics in biological systems.
摘要
我们使用共振能量转移和频域荧光测定法来测量粘性介质中供体到受体的缓慢扩散。频域共振能量转移数据使用一种新的数值算法进行分析，该算法用于预测在供体衰减时间内发生扩散时供体强度的衰减。通过使用铼金属 - 配体配合物作为具有微秒级衰减时间的供体，我们能够测量低至2×10⁻⁹cm²/s的供体与受体之间的相互扩散系数。具有微秒级衰减时间的发光体的可用性以及适当的理论表明，可利用扩散增强能量转移来测量生物系统中的扩散过程和结构动力学。
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