可调节的GAL1启动子使Erg11p过表达，从而赋予酿酒酵母对氟康唑的抗性。
Overexpression of Erg11p by the regulatable GAL1 promoter confers fluconazole resistance in Saccharomyces cerevisiae.
作者信息
Kontoyiannis D P, Sagar N, Hirschi K D
机构信息
Department of Internal Medicine Specialties, Section of Infectious Diseases, The University of Texas M. D. Anderson Cancer Center, Houston, Texas, USA.
出版信息
Antimicrob Agents Chemother. 1999 Nov;43(11):2798-800. doi: 10.1128/AAC.43.11.2798.
Abstract
The contribution of the dosage of target enzyme P-450 14alpha-demethylase (14alphaDM) to fluconazole resistance in both Candida albicans and Saccharomyces cerevisiae remains unclear. Here, we show that overexpression of Saccharomyces P-450 14alphaDM in S. cerevisiae, under the control of the regulatable promoter GAL1, results in azole resistance.
摘要
目标酶P-450 14α-脱甲基酶（14αDM）的剂量对白色念珠菌和酿酒酵母中氟康唑耐药性的影响仍不清楚。在此，我们表明，在可调节启动子GAL1的控制下，酿酒酵母中酿酒酵母P-450 14αDM的过表达会导致唑类耐药。
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