HeLa细胞中的短寿命信使核糖核酸及其对细胞质聚腺苷酸积累动力学的影响。
Short-lived messenger RNA in HeLa cells and its impace on the kinetics of accumulation of cytoplasmic polyadenylate.
作者信息
Puckett L, Chambers S, Darnell J E
出版信息
Proc Natl Acad Sci U S A. 1975 Jan;72(1):389-93. doi: 10.1073/pnas.72.1.389.
Abstract
Accumulation of [3H]adenine in the acid-soluble pool and in nuclear and cytoplasmic poly(A) of HeLa cells shows that the nuclear poly(A) rises along a curve similar to that of the acid-soluble pool. By use of a [3H]guanosine pulse-chase experiment in adenine-grown cells, at least 35-50% of the pulse-labeled mRNA was found to have a half-life of about 1-2 hr. A mathematical model involving nuclear poly(A) synthesis and conservative transport to the cytoplasm has been derived from the new information about mRNA with a short half-life. This model predicts curves similar to those found for nuclear and cytoplasmic accumulation of poly(A). Thus, there is no necessity on kinetic grounds to invoke either nuclear turnover or cytoplasmic synthesis of poly(A).
摘要
[3H]腺嘌呤在海拉细胞酸溶性池以及细胞核和细胞质多聚腺苷酸中的积累表明，细胞核多聚腺苷酸沿着与酸溶性池相似的曲线上升。通过在腺嘌呤培养的细胞中进行[3H]鸟苷脉冲追踪实验，发现至少35 - 50%的脉冲标记mRNA的半衰期约为1 - 2小时。基于关于半衰期较短的mRNA的新信息，推导出了一个涉及细胞核多聚腺苷酸合成以及向细胞质保守转运的数学模型。该模型预测的曲线与多聚腺苷酸在细胞核和细胞质中的积累曲线相似。因此，从动力学角度来看，没有必要援引细胞核周转或细胞质中多聚腺苷酸合成的观点。
相似文献
1
Short-lived messenger RNA in HeLa cells and its impace on the kinetics of accumulation of cytoplasmic polyadenylate.HeLa细胞中的短寿命信使核糖核酸及其对细胞质聚腺苷酸积累动力学的影响。
Proc Natl Acad Sci U S A. 1975 Jan;72(1):389-93. doi: 10.1073/pnas.72.1.389.
2
Differential metabolism of large and small poly(A) sequences in the heterogeneous nuclear RNA of HeLa cells.HeLa细胞异质性核RNA中大小不同的聚腺苷酸序列的差异代谢
Proc Natl Acad Sci U S A. 1974 Jan;71(1):200-4. doi: 10.1073/pnas.71.1.200.
3
Polyadenylate metabolism in the nuclei and cytoplasm of Saccharomyces cerevisiae.酿酒酵母细胞核与细胞质中的聚腺苷酸代谢
J Biol Chem. 1975 Jul 25;250(14):5640-6.
4
Metabolism of the polyadenylate sequence of nuclear RNA and messenger RNA in mammalian cells.哺乳动物细胞核RNA和信使RNA聚腺苷酸序列的代谢
Cell. 1975 Jul;5(3):271-80. doi: 10.1016/0092-8674(75)90102-6.
5
Turnover of myeloma messenger DNA.骨髓瘤信使核糖核酸的周转
Biochem Biophys Res Commun. 1973 Jun 19;52(4):1483-91. doi: 10.1016/0006-291x(73)90668-2.
6
The role of cytoplasmic membranes in controlling the transport of nuclear messenger RNA and initiation of protein synthesis.细胞质膜在控制核信使核糖核酸运输及蛋白质合成起始过程中的作用。
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2658-62. doi: 10.1073/pnas.71.7.2658.
7
Essential factors in the kinetic analysis of RNA synthesis in HeLa cells.HeLa细胞中RNA合成动力学分析的关键因素。
J Cell Physiol. 1977 Mar;90(3):521-34. doi: 10.1002/jcp.1040900315.
8
Polyadenylic acid segment in mRNA becomes shorter with age.信使核糖核酸中的聚腺苷酸片段会随着年龄增长而变短。
Nat New Biol. 1973 Feb 28;241(113):265-8. doi: 10.1038/newbio241265a0.
9
Possible relationship of poly(A) shortening to mRNA turnover.多聚腺苷酸缩短与信使核糖核酸周转的可能关系。
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1077-81. doi: 10.1073/pnas.72.3.1077.
10
The messenger-like properties of the poly(A)plus RNA in mammalian mitochondria.哺乳动物线粒体中多聚腺苷酸（poly(A)）加尾RNA的信使样特性。
Cell. 1974 Dec;3(4):335-9. doi: 10.1016/0092-8674(74)90047-6.
引用本文的文献
1
mA mRNA modifications are deposited in nascent pre-mRNA and are not required for splicing but do specify cytoplasmic turnover.mA信使核糖核酸修饰存在于新生的前体信使核糖核酸中，剪接过程并不需要这些修饰，但它们确实决定了细胞质中的周转情况。
Genes Dev. 2017 May 15;31(10):990-1006. doi: 10.1101/gad.301036.117.
2
Determination of in vivo RNA kinetics using RATE-seq.使用RATE-seq测定体内RNA动力学。
RNA. 2014 Oct;20(10):1645-52. doi: 10.1261/rna.045104.114. Epub 2014 Aug 26.
3
Reflections on the history of pre-mRNA processing and highlights of current knowledge: a unified picture.对前体 mRNA 处理历史的反思及当前知识要点：一幅统一的画面。
RNA. 2013 Apr;19(4):443-60. doi: 10.1261/rna.038596.113. Epub 2013 Feb 25.
4
Decay rates of human mRNAs: correlation with functional characteristics and sequence attributes.人类mRNA的降解速率：与功能特征和序列属性的相关性
Genome Res. 2003 Aug;13(8):1863-72. doi: 10.1101/gr.1272403.
5
Glucocorticoids regulate proopiomelanocortin gene expression in vivo at the levels of transcription and secretion.糖皮质激素在转录和分泌水平上调节体内阿片促黑皮质素原基因的表达。
Proc Natl Acad Sci U S A. 1983 Nov;80(22):6982-6. doi: 10.1073/pnas.80.22.6982.
6
Order of polyadenylic acid addition and splicing events in early adenovirus mRNA formation.早期腺病毒信使核糖核酸形成过程中多聚腺苷酸添加和剪接事件的顺序
J Virol. 1980 Jan;33(1):286-91. doi: 10.1128/JVI.33.1.286-291.1980.
7
Regulation of c-myc mRNA stability in vitro by a labile destabilizer with an essential nucleic acid component.一种具有必需核酸成分的不稳定去稳定剂在体外对c-myc mRNA稳定性的调控。
Mol Cell Biol. 1989 May;9(5):1996-2006. doi: 10.1128/mcb.9.5.1996-2006.1989.
8
Three mRNA populations differing in turnover and processing in mouse liver.小鼠肝脏中在周转和加工方面存在差异的三种mRNA群体。
Mol Biol Rep. 1975 Dec;2(4):269-75. doi: 10.1007/BF00357013.
9
Short-lived methylated messenger RNA in mouse kidney.小鼠肾脏中的短寿命甲基化信使核糖核酸
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2609-13. doi: 10.1073/pnas.73.8.2609.
10
Very short-lived and stable mRNAs from resting human lymphocytes.来自静息人类淋巴细胞的极短寿命且稳定的信使核糖核酸。
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3873-7. doi: 10.1073/pnas.72.10.3873.
本文引用的文献
1
Utilization of precurosors for nucleic acid synthesis by human cell cultures.人类细胞培养物对核酸合成前体的利用
Arch Biochem Biophys. 1959 Sep;84:143-50. doi: 10.1016/0003-9861(59)90563-6.
2
ENTRANCE OF NEWLY FORMED MESSENGER RNA AND RIBOSOMES INTO HELA CELL CYTOPLASM.新形成的信使核糖核酸和核糖体进入海拉细胞细胞质。
J Mol Biol. 1965 Feb;11:187-201. doi: 10.1016/s0022-2836(65)80050-x.
3
4
Amino acid metabolism in mammalian cell cultures.哺乳动物细胞培养中的氨基酸代谢
Science. 1959 Aug 21;130(3373):432-7. doi: 10.1126/science.130.3373.432.
5
Rapidly labeled HeLa cell nuclear RNA. I. Identification by zone sedimentation of a heterogeneous fraction separate from ribosomal precursor RNA.快速标记的海拉细胞核RNA。I. 通过区带沉降法鉴定与核糖体前体RNA分离的异质组分。
J Mol Biol. 1966 Aug;19(2):349-61. doi: 10.1016/s0022-2836(66)80009-8.
6
RNA metabolism in the HeLa cell nucleus.海拉细胞核中的RNA代谢
J Mol Biol. 1966 May;17(1):117-30. doi: 10.1016/s0022-2836(66)80098-0.
7
Free nucleotides in animal tissues.动物组织中的游离核苷酸。
Prog Nucleic Acid Res Mol Biol. 1964;3:299-334. doi: 10.1016/s0079-6603(08)60744-8.
8
Polyadenylic acid sequences: role in conversion of nuclear RNA into messenger RNA.聚腺苷酸序列：在核RNA转变为信使RNA过程中的作用
Science. 1971 Oct 29;174(4008):507-10. doi: 10.1126/science.174.4008.507.
9
Synthesis and turnover of nuclear and cytoplasmic polyadenylic acid in mouse L cells.小鼠L细胞中核内和胞质多聚腺苷酸的合成与周转
J Mol Biol. 1974 Jan 25;82(3):315-31. doi: 10.1016/0022-2836(74)90593-2.
10
Messenger RNA turnover in mouse L cells.小鼠L细胞中的信使核糖核酸周转
J Mol Biol. 1973 Oct 5;79(4):681-96. doi: 10.1016/0022-2836(73)90071-5.
Zotero 插件