多聚腺苷酸缩短与信使核糖核酸周转的可能关系。
Possible relationship of poly(A) shortening to mRNA turnover.
作者信息
Sheiness D, Puckett L, Darnell J E
出版信息
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1077-81. doi: 10.1073/pnas.72.3.1077.
Abstract
Whereas the original size of poly(A) in HeLa cells is about 200 nucleotides, at steady state most of the poly(A) in mRNA contains less than 50 nucleotides. An endonucleolytic attack on poly(A) is suggested as the most likely method to accumulate short pieces of poly(A). Both poly(A) shortening and mRNA turnover appear to be inhibited by emetine, a drug that stops translation. It is possible that a random endonucleolytic attack leads to scission of poly(A) to a size below which the mRNA is unstable.
摘要
虽然HeLa细胞中聚腺苷酸（poly(A)）的原始长度约为200个核苷酸，但在稳态时，mRNA中的大多数聚腺苷酸所含核苷酸少于50个。对聚腺苷酸的内切核酸酶攻击被认为是积累短片段聚腺苷酸的最可能方式。聚腺苷酸缩短和mRNA周转似乎都受到放线菌酮的抑制，放线菌酮是一种能阻止翻译的药物。有可能随机的内切核酸酶攻击导致聚腺苷酸断裂成mRNA不稳定的长度以下。
相似文献
1
Possible relationship of poly(A) shortening to mRNA turnover.多聚腺苷酸缩短与信使核糖核酸周转的可能关系。
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1077-81. doi: 10.1073/pnas.72.3.1077.
2
Polyadenylic acid segment in mRNA becomes shorter with age.信使核糖核酸中的聚腺苷酸片段会随着年龄增长而变短。
Nat New Biol. 1973 Feb 28;241(113):265-8. doi: 10.1038/newbio241265a0.
3
Differential metabolism of large and small poly(A) sequences in the heterogeneous nuclear RNA of HeLa cells.HeLa细胞异质性核RNA中大小不同的聚腺苷酸序列的差异代谢
Proc Natl Acad Sci U S A. 1974 Jan;71(1):200-4. doi: 10.1073/pnas.71.1.200.
4
Short-lived messenger RNA in HeLa cells and its impace on the kinetics of accumulation of cytoplasmic polyadenylate.HeLa细胞中的短寿命信使核糖核酸及其对细胞质聚腺苷酸积累动力学的影响。
Proc Natl Acad Sci U S A. 1975 Jan;72(1):389-93. doi: 10.1073/pnas.72.1.389.
5
Metabolism of the polyadenylate sequence of nuclear RNA and messenger RNA in mammalian cells.哺乳动物细胞核RNA和信使RNA聚腺苷酸序列的代谢
Cell. 1975 Jul;5(3):271-80. doi: 10.1016/0092-8674(75)90102-6.
6
The metabolism of a poly(A) minus mRNA fraction in HeLa cells.海拉细胞中多聚腺苷酸缺失mRNA组分的代谢
Cell. 1974 Sep;3(1):1-10. doi: 10.1016/0092-8674(74)90030-0.
7
Polyadenylic acid sequences: role in conversion of nuclear RNA into messenger RNA.聚腺苷酸序列：在核RNA转变为信使RNA过程中的作用
Science. 1971 Oct 29;174(4008):507-10. doi: 10.1126/science.174.4008.507.
8
The messenger-like properties of the poly(A)plus RNA in mammalian mitochondria.哺乳动物线粒体中多聚腺苷酸（poly(A)）加尾RNA的信使样特性。
Cell. 1974 Dec;3(4):335-9. doi: 10.1016/0092-8674(74)90047-6.
9
Translation of mouse globin messenger ribonucleic acid from which the poly(adenylic acid) sequence has been removed.已去除聚腺苷酸序列的小鼠珠蛋白信使核糖核酸的翻译产物。
Biochemistry. 1974 Feb 12;13(4):703-7. doi: 10.1021/bi00701a011.
10
Poly(adenylic acid) sequences in adenovirus ribonucleic acid released from isolated nuclei.从分离细胞核中释放的腺病毒核糖核酸中的聚腺苷酸序列。
Biochemistry. 1973 Feb 27;12(5):920-5. doi: 10.1021/bi00729a020.
引用本文的文献
1
Rapid nuclear deadenylation of mammalian messenger RNA.哺乳动物信使核糖核酸的快速核去腺苷酸化
iScience. 2022 Dec 28;26(1):105878. doi: 10.1016/j.isci.2022.105878. eCollection 2023 Jan 20.
2
Roles of mRNA poly(A) tails in regulation of eukaryotic gene expression.mRNA 多聚（A）尾在真核基因表达调控中的作用。
Nat Rev Mol Cell Biol. 2022 Feb;23(2):93-106. doi: 10.1038/s41580-021-00417-y. Epub 2021 Sep 30.
3
Transcriptional and posttranscriptional events associated with neural maturation.与神经成熟相关的转录和转录后事件。
Neurochem Res. 1976 Dec;1(6):557-72. doi: 10.1007/BF00965598.
4
Oligoadenylate is present in the mitochondrial RNA of Saccharomyces cerevisiae.寡腺苷酸存在于酿酒酵母的线粒体RNA中。
Mol Cell Biol. 1982 Apr;2(4):450-6. doi: 10.1128/mcb.2.4.450-456.1982.
5
Newly formed mRNA lacking polyadenylic acid enters the cytoplasm and the polyribosomes but has a shorter half-life in the absence of polyadenylic acid.新形成的缺乏聚腺苷酸的信使核糖核酸进入细胞质和多核糖体，但在缺乏聚腺苷酸的情况下半衰期较短。
Mol Cell Biol. 1982 May;2(5):517-25. doi: 10.1128/mcb.2.5.517-525.1982.
6
Addition of poly(A) to nuclear RNA occurs soon after RNA synthesis.核RNA合成后不久就会添加多聚腺苷酸（poly(A)）。
J Cell Biol. 1980 Sep;86(3):844-8. doi: 10.1083/jcb.86.3.844.
7
In vitro protein synthesis activity of poly(A) RNA from neuroblastoma cells.神经母细胞瘤细胞中聚腺苷酸（poly(A)）RNA的体外蛋白质合成活性。
Neurochem Res. 1980 Mar;5(3):271-9. doi: 10.1007/BF00964615.
8
Messenger RNA stability in Saccharomyces cerevisiae: the influence of translation and poly(A) tail length.酿酒酵母中的信使核糖核酸稳定性：翻译和聚腺苷酸尾长度的影响
Nucleic Acids Res. 1987 Mar 25;15(6):2417-29. doi: 10.1093/nar/15.6.2417.
9
Poly(A) shortening and degradation of the 3' A+U-rich sequences of human c-myc mRNA in a cell-free system.在无细胞体系中，人c-myc mRNA的多聚腺苷酸（Poly(A)）缩短及3'端富含A+U序列的降解
Mol Cell Biol. 1988 Apr;8(4):1697-708. doi: 10.1128/mcb.8.4.1697-1708.1988.
10
Adenovirus E1B proteins are required for accumulation of late viral mRNA and for effects on cellular mRNA translation and transport.腺病毒E1B蛋白是晚期病毒mRNA积累以及影响细胞mRNA翻译和转运所必需的。
Mol Cell Biol. 1985 Oct;5(10):2552-8. doi: 10.1128/mcb.5.10.2552-2558.1985.
本文引用的文献
1
Structural basis for inhibition of protein synthesis by emetine and cycloheximide based on an analogy between ipecac alkaloids and glutarimide antibiotics.基于吐根生物碱与戊二酰亚胺类抗生素之间的类比，阐明吐根碱和放线菌酮抑制蛋白质合成的结构基础。
Proc Natl Acad Sci U S A. 1966 Dec;56(6):1867-74. doi: 10.1073/pnas.56.6.1867.
2
Amino acid metabolism in mammalian cell cultures.哺乳动物细胞培养中的氨基酸代谢
Science. 1959 Aug 21;130(3373):432-7. doi: 10.1126/science.130.3373.432.
3
Nucleotide sequence of KB cell 5S RNA.KB细胞5S核糖核酸的核苷酸序列。
Science. 1967 Dec 29;158(3809):1695-9. doi: 10.1126/science.158.3809.1695.
4
Nucleotide sequence of an RNA from cells infected with adenovirus 2.来自感染腺病毒2的细胞的一种RNA的核苷酸序列。
Science. 1970 Feb 6;167(3919):879-81. doi: 10.1126/science.167.3919.879.
5
The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.用聚丙烯酰胺凝胶电泳法分离高分子量核糖核酸
Biochem J. 1967 Jan;102(1):251-7. doi: 10.1042/bj1020251.
6
Biogenesis and characterization of histone messenger RNA in HeLa cells.HeLa细胞中组蛋白信使核糖核酸的生物合成与特性分析
J Mol Biol. 1972 Jun 28;67(3):397-406. doi: 10.1016/0022-2836(72)90458-5.
7
The isolation and purification of rapidly labeled polysome-bound ribonucleic acid on polythymidylate cellulose.
J Biol Chem. 1972 May 25;247(10):3365-7.
8
Messenger RNA turnover in mouse L cells.小鼠L细胞中的信使核糖核酸周转
J Mol Biol. 1973 Oct 5;79(4):681-96. doi: 10.1016/0022-2836(73)90071-5.
9
Absence of polyadenylic acid from reovirus messenger ribonucleic acid.呼肠孤病毒信使核糖核酸中不存在聚腺苷酸。
J Biol Chem. 1973 Dec 10;248(23):7993-8.
10
Messenger RNA in HeLa cells: kinetics of formation and decay.海拉细胞中的信使核糖核酸：合成与降解动力学
J Mol Biol. 1973 Aug 5;78(2):321-34. doi: 10.1016/0022-2836(73)90119-8.
Zotero 插件