呼肠孤病毒信使核糖核酸5'末端的m7G5'ppp5'GmptcpUp
m7G5'ppp5'GmptcpUp at the 5' terminus of reovirus messenger RNA.
作者信息
Faust M, Hastings K E, Millward S
出版信息
Nucleic Acids Res. 1975 Aug;2(8):1329-43. doi: 10.1093/nar/2.8.1329.
Abstract
In the presence of S-adenosyl methionine the 5' terminal guanosine residue of in vitro synthesized reovirus mRNA becomes methylated at the 2'-OH position. In addition, 7-methyl guanylic acid is condensed covalently at the 5' terminus resulting in the formation of a 5' to 5' triphosphate bridge. Analysis of the 5' terminal sequence of methylated reovirus mRNA revealed that it has the structure m7G5'ppp5'GmpCpUp.
摘要
在S-腺苷甲硫氨酸存在的情况下，体外合成的呼肠孤病毒mRNA的5'末端鸟苷残基在2'-OH位置发生甲基化。此外，7-甲基鸟苷酸在5'末端共价缩合，导致形成5'至5'三磷酸桥。对甲基化呼肠孤病毒mRNA的5'末端序列分析表明，其结构为m7G5'ppp5'GmpCpUp。
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