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通过16S rRNA基因的PCR-限制性片段长度多态性分析快速鉴定弯曲杆菌、弓形杆菌和幽门螺杆菌分离株。

Rapid identification of Campylobacter, Arcobacter, and Helicobacter isolates by PCR-restriction fragment length polymorphism analysis of the 16S rRNA gene.

作者信息

Marshall S M, Melito P L, Woodward D L, Johnson W M, Rodgers F G, Mulvey M R

机构信息

National Research Council Canada, Institute for Biological Sciences, Ottawa, Ontario K1A 0R6, Canada.

出版信息

J Clin Microbiol. 1999 Dec;37(12):4158-60. doi: 10.1128/JCM.37.12.4158-4160.1999.

DOI:10.1128/JCM.37.12.4158-4160.1999
PMID:10565952
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC85910/
Abstract

A rapid two-step identification scheme based on PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the 16S rRNA gene was developed in order to differentiate isolates belonging to the Campylobacter, Arcobacter, and Helicobacter genera. For 158 isolates (26 reference cultures and 132 clinical isolates), specific RFLP patterns were obtained and species were successfully identified by this assay.

摘要

为了区分弯曲杆菌属、弓形杆菌属和螺杆菌属的分离株，开发了一种基于16S rRNA基因的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析的快速两步鉴定方案。对于158株分离株（26株参考培养物和132株临床分离株），获得了特定的RFLP模式，并通过该检测成功鉴定了菌种。

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本文引用的文献

1
Strategy for the detection of Helicobacter species by amplification of 16S rRNA genes and identification of H. felis in a human gastric biopsy.通过扩增16S rRNA基因检测幽门螺杆菌属以及在人胃活检中鉴定猫幽门螺杆菌的策略。
Res Microbiol. 1997 May;148(4):315-26. doi: 10.1016/S0923-2508(97)81587-2.
2
PCR detection, identification to species level, and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples.直接从腹泻样本中对空肠弯曲菌和结肠弯曲菌进行PCR检测、种水平鉴定及指纹图谱分析。
J Clin Microbiol. 1997 Oct;35(10):2568-72. doi: 10.1128/jcm.35.10.2568-2572.1997.
3
Rapid identification by PCR of the genus Campylobacter and of five Campylobacter species enteropathogenic for man and animals.通过聚合酶链反应（PCR）快速鉴定弯曲杆菌属以及对人和动物具有致病性的五种弯曲杆菌。
Res Microbiol. 1996 Nov-Dec;147(9):707-18. doi: 10.1016/s0923-2508(97)85118-2.
4
A molecular scheme based on 23S rRNA gene polymorphisms for rapid identification of Campylobacter and Arcobacter species.一种基于23S rRNA基因多态性的分子方案，用于快速鉴定弯曲杆菌属和弓形杆菌属菌种。
J Clin Microbiol. 1997 Sep;35(9):2401-4. doi: 10.1128/jcm.35.9.2401-2404.1997.
5
RAPD analysis of environmental, food and clinical isolates of Campylobacter spp.弯曲杆菌属环境、食品及临床分离株的随机扩增多态性DNA分析
FEMS Immunol Med Microbiol. 1997 Jun;18(2):119-24. doi: 10.1111/j.1574-695X.1997.tb01036.x.
6
Identification of Arcobacter isolates by PCR.通过聚合酶链反应鉴定弓形杆菌分离株。
Lett Appl Microbiol. 1996 Oct;23(4):241-4. doi: 10.1111/j.1472-765x.1996.tb00074.x.
7
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8
Identification methods for campylobacters, helicobacters, and related organisms.弯曲杆菌、螺杆菌及相关微生物的鉴定方法。
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9
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10
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Microbiology (Reading). 1996 Jan;142 ( Pt 1):3-16. doi: 10.1099/13500872-142-1-3.