Al-Khateeb S F, Barkas T
Clin Exp Immunol. 1978 Dec;34(3):429-35.
A simple and sensitive method for the detection and quantification of soluble immune complexes in sera has been developed, by utilizing Fc receptors on rat spleen leucocyte (RSL). Aggregated human IgG (AHG) was used as an model of immune complexes and its uptake by RSL was quantified using I-anti-human IgG. RSL were found to bind AHG in medium as well as in ethylenediamine tetra-acetic acid (EDTA) inactivated and absorbed serum. The limit of detection of this method was 2 μg AHG/ml in medium and 4 μg AHG/ml in serum. Sera samples from patients with thyroid disorders and from diabetics were incubated with RSL and the cells incubated with I-anti-human IgG. The amount of label bound to the cells was determined and referred to a standard curve of radiolabelled antibody uptake by cells previously incubated with varying amounts of AHG in serum. The results obtained were compared with those obtained using the Raji-cell radioimmunoassay. Human anti-rat erythrocyte antibody was detected in normal human serum. The amount of complexes formed in serum by heat treatment was quantified by this method.
通过利用大鼠脾脏白细胞（RSL）上的Fc受体，已开发出一种用于检测和定量血清中可溶性免疫复合物的简单且灵敏的方法。聚集的人IgG（AHG）用作免疫复合物的模型，其被RSL摄取的情况使用I-抗人IgG进行定量。发现RSL在培养基以及乙二胺四乙酸（EDTA）灭活和吸收的血清中均能结合AHG。该方法在培养基中的检测限为2μg AHG/ml，在血清中的检测限为4μg AHG/ml。将甲状腺疾病患者和糖尿病患者的血清样本与RSL一起孵育，然后将细胞与I-抗人IgG一起孵育。测定与细胞结合的标记量，并参照先前在血清中用不同量的AHG孵育的细胞摄取放射性标记抗体的标准曲线。将获得的结果与使用Raji细胞放射免疫测定法获得的结果进行比较。在正常人血清中检测到了人抗大鼠红细胞抗体。通过该方法对热处理后血清中形成的复合物量进行了定量。
