利用巨噬细胞Fc受体摄取可溶性免疫复合物进行放射分析。
Radioassay of soluble immune complexes using their uptake by macrophage Fc receptors.
作者信息
Kávai M, Dankó K, Kalmár E, Francia I, Szegedi G
出版信息
Immunology. 1977 May;32(5):617-21.
Abstract
Sera from patients were tested for the presence of soluble immune complexes (IC) by a method based on the observation that IC inhibit uptake of aggregated IgG by the Fc receptors of macrophages. From the saturation curve of macrophages with 125I-labelled aggregates the optimal inhibitory effect of a measured amount of IC was determined. This radioassay is highly sensitive, being capable of detecting 20 ng of IC, is reproducible and covers a wide range of measurement (20-2000 ng).
摘要
通过一种基于免疫复合物（IC）抑制巨噬细胞Fc受体摄取聚集IgG这一观察结果的方法，检测患者血清中可溶性免疫复合物（IC）的存在。根据用125I标记的聚集体对巨噬细胞的饱和曲线，确定一定量IC的最佳抑制效果。这种放射测定法高度灵敏，能够检测到20 ng的IC，具有可重复性，并且测量范围广泛（20 - 2000 ng）。
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