Ng L K, Mulvey M R, Martin I, Peters G A, Johnson W
Bureau of Microbiology, Laboratory Centre for Disease Control, Health Canada, Winnipeg, Manitoba, Canada.
Antimicrob Agents Chemother. 1999 Dec;43(12):3018-21. doi: 10.1128/AAC.43.12.3018.
PCR was used to identify antibiotic resistance determinants in 31 Canadian Salmonella serovar Typhimurium DT104 isolates. Genes encoding resistance to ampicillin (pse1 or blaP1), chloramphenicol (pasppflo-like), streptomycin-spectinomycin (aadA2), sulfonamide (sulI), and tetracycline [tet(G)] were mapped to a 13-kb region of DNA of one isolate. Two copies of sulI were identified and mapped to the 3' end of either pse1 or aadA2 integrons. The two integrons were separated by the pasppflo-like gene and the tet(G) gene. The kanamycin resistance determinant (aphA-1) was present on a 2.0-MDa plasmid (five isolates) or on the chromosome (three isolates).
采用聚合酶链反应（PCR）对31株加拿大鼠伤寒沙门氏菌DT104分离株中的抗生素耐药决定因素进行鉴定。编码对氨苄西林（pse1或blaP1）、氯霉素（pasppflo样）、链霉素-壮观霉素（aadA2）、磺胺类药物（sulI）和四环素[tet(G)]耐药的基因被定位到一个分离株的13kb DNA区域。鉴定出两个sulI拷贝，并将其定位到pse1或aadA2整合子的3'端。两个整合子被pasppflo样基因和tet(G)基因隔开。卡那霉素耐药决定因素（aphA-1）存在于2.0兆道尔顿的质粒上（5株分离株）或染色体上（3株分离株）。
