Power P, Radice M, Barberis C, de Mier C, Mollerach M, Maltagliatti M, Vay C, Famiglietti A, Gutkind G
Departamento de Microbiología, Immunología y Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Eur J Clin Microbiol Infect Dis. 1999 Oct;18(10):743-7. doi: 10.1007/s100960050391.
The frequency of enterobacterial isolates with high resistance to expanded-spectrum beta-lactam antibiotics (mainly cefotaxime or ceftriaxone) has increased notoriously in Argentina, mainly because of the spread of extended-spectrum beta-lactamases. The aim of this work was the study of extended-spectrum beta-lactamases in several Morganella morganii isolates with unusually high resistance to ceftriaxone. These strains produced at least two beta-lactamases, of apparent pIs of 5.4 and 8.2, molecular weight 23 000, well inhibited by clavulanate, compatible with a broad-spectrum beta-lactamase - perhaps TEM-1 - and an extended-spectrum beta-lactamase, respectively. The extended-spectrum beta-lactamase was identified as a CTX-M-type beta-lactamase - probably CTX-M-2 - by polymerase chain reaction, restriction profile analysis and DNA-DNA hybridisation. The remaining isolates studied produced either the broad-spectrum beta-lactamase plus the ubiquitous AmpC beta-lactamase (13 strains), or the AmpC beta-lactamase only (10 strains).
在阿根廷，对超广谱β-内酰胺类抗生素（主要是头孢噻肟或头孢曲松）具有高耐药性的肠杆菌分离株的频率显著增加，主要原因是超广谱β-内酰胺酶的传播。这项工作的目的是研究几种对头孢曲松具有异常高耐药性的摩根氏摩根菌分离株中的超广谱β-内酰胺酶。这些菌株产生了至少两种β-内酰胺酶，表观pI分别为5.4和8.2，分子量为23000，克拉维酸能很好地抑制它们，分别与一种广谱β-内酰胺酶（可能是TEM-1）和一种超广谱β-内酰胺酶相符。通过聚合酶链反应、限制性图谱分析和DNA-DNA杂交，将超广谱β-内酰胺酶鉴定为CTX-M型β-内酰胺酶——可能是CTX-M-2。其余研究的分离株要么产生广谱β-内酰胺酶加普遍存在的AmpCβ-内酰胺酶（13株），要么仅产生AmpCβ-内酰胺酶（10株）。
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