Perilli M, Segatore B, de Massis M R, Riccio M L, Bianchi C, Zollo A, Rossolini G M, Amicosante G
Dipartimento di Scienze e Tecnologie Biomediche, Cattedra di Enzimologia, Università di L'Aquila, Italy.
Antimicrob Agents Chemother. 2000 Sep;44(9):2537-9. doi: 10.1128/AAC.44.9.2537-2539.2000.
A new natural TEM-2 derivative, named TEM-72, was identified in a Proteus mirabilis strain and in a Morganella morganii strain isolated in Italy in 1999. Compared to TEM-1, TEM-72 contains the following amino acid substitutions: Q39K, M182T, G238S, and E240K. Kinetic analysis showed that TEM-72 exhibits an extended-spectrum activity, including activity against oxyimino-cephalosporins and aztreonam. Expression of bla(TEM-72) in Escherichia coli was capable of decreasing the host susceptibility to the above drugs.
1999年在意大利分离出的一株奇异变形杆菌和一株摩根摩根菌中鉴定出一种新的天然TEM-2衍生物，命名为TEM-72。与TEM-1相比，TEM-72包含以下氨基酸取代：Q39K、M182T、G238S和E240K。动力学分析表明，TEM-72具有广谱活性，包括对氧亚氨基头孢菌素和氨曲南的活性。bla(TEM-72)在大肠杆菌中的表达能够降低宿主对上述药物的敏感性。
