Nir U, Cohen B, Chen L, Revel M
Nucleic Acids Res. 1984 Sep 25;12(18):6979-93. doi: 10.1093/nar/12.18.6979.
Induction of IFN-beta 1 RNA was studied in the mouse cell line SR117-21E transformed by a BPV episome containing the human IFN-beta 1 gene deleted of promoter sequences upstream from position -40. Nuclei isolated from these cells synthesize constitutively IFN-beta 1 RNA from the partially deleted promoter. The IFN-beta 1 RNA synthesized by nuclei of uninduced SR117-21E cells is similar to that made by nuclei of poly(rI):(rC)-induced cells, but does not accumulate and hence no IFN is produced unless the cells have been treated either by ds RNA or by cycloheximide. We conclude that the IFN-beta 1 gene has, in addition to the transcription control due to upstream promoter sequences, an additional post-transcriptional control acting on mRNA accumulation and linked to sequences close to the TATA box and RNA start site. Both controls are relieved by ds RNA.
在由含有缺失了-40位上游启动子序列的人IFN-β1基因的BPV附加体转化的小鼠细胞系SR117-21E中，研究了IFN-β1 RNA的诱导情况。从这些细胞中分离出的细胞核组成性地从部分缺失的启动子合成IFN-β1 RNA。未诱导的SR117-21E细胞核合成的IFN-β1 RNA与聚（rI）:（rC）诱导的细胞核合成的相似，但不会积累，因此除非细胞用双链RNA或环己酰亚胺处理，否则不会产生IFN。我们得出结论，IFN-β1基因除了因上游启动子序列产生转录控制外，还有一种额外的转录后控制作用于mRNA积累，并与靠近TATA盒和RNA起始位点的序列相关。两种控制都可被双链RNA解除。
