Sogin S J, Saunders C A
J Bacteriol. 1980 Oct;144(1):74-81. doi: 10.1128/jb.144.1.74-81.1980.
Stationary-phase cells of Saccharomyces cerevisiae were found to have a reduced polyadenylate [poly(A)] content as compared with exponential-phase cells. A sizing procedure for poly(A) was devised to distinguish between alternative hypotheses to explain this reduction. Two major size classes of poly(A) were found. The decreased representation of the larger of the two classes accounted for the majority of the poly(A) loss. The remainder of the loss was accounted for by fewer poly(A)-containing sequences. The smaller of the two poly(A) classes was apparently not of mitochondrial origin and may be added transcriptionally.
与指数生长期的细胞相比,酿酒酵母的稳定期细胞被发现具有降低的聚腺苷酸[poly(A)]含量。设计了一种用于poly(A)的分级程序,以区分用于解释这种降低的替代假说。发现了两种主要大小类别的poly(A)。两类中较大的那一类的减少占了poly(A)损失的大部分。损失的其余部分是由含poly(A)序列较少所致。两种poly(A)类别中较小的那一类显然不是线粒体起源的,可能是转录添加的。