关于小鼠艾氏腹水瘤细胞对白喉毒素的所谓高敏感性。
On the alleged high sensitivity of mouse Ehrlich-Lettre ascites tumor cells to diphtheria toxin.
作者信息
Pappenheimer A M, Randall V
出版信息
Proc Natl Acad Sci U S A. 1975 Aug;72(8):3149-52. doi: 10.1073/pnas.72.8.3149.
It was recently reported by Iglewski and Rittenberg in THESE PROCEEDINGS (71, 2707-2710, 1974) that low doses of purified diphtheria toxin inhibit protein synthesis in mouse Ehrlich-Lettre ascites carcinoma cells cultured in vitro. These observations could not be confirmed by us nor could the authors' further claim that toxin can cause regression of well-established ascites tumors in preimmunized mice be confirmed. Although temporary regression of such tumors can be demonstrated in unimmunized mice following intraperitoneal injection of diphtheria toxin, the amounts of toxin required are high and approach the lethal dose. About the same amount of CRM45, a tox gene product serologically related to diphtheria toxin but only 1/10,000th as toxic for guinea pigs, will also cause temporary regression in tumor-bearing mice.
伊格莱夫斯基和里滕伯格最近在《这些论文集》（第71卷，2707 - 2710页，1974年）中报道，低剂量的纯化白喉毒素可抑制体外培养的小鼠艾氏腹水癌细胞中的蛋白质合成。我们无法证实这些观察结果，作者进一步声称毒素可使预先免疫的小鼠体内已形成的腹水肿瘤消退这一说法也无法得到证实。尽管在未免疫的小鼠腹腔注射白喉毒素后可证明此类肿瘤出现暂时消退，但所需的毒素量很高，接近致死剂量。约等量的CRM45，一种与白喉毒素血清学相关但对豚鼠的毒性仅为其万分之一的毒素基因产物，也会使荷瘤小鼠出现暂时消退。
Zotero 插件