Ferreira A C, Nobre M F, Moore E, Rainey F A, Battista J R, da Costa M S
Departamento de Bioquímica Universidade de Coimbra, Portugal.
Extremophiles. 1999 Nov;3(4):235-8. doi: 10.1007/s007920050121.
In this study we characterized new strains of the slightly thermophilic species Rubrobacter radiotolerans and the thermophilic species Rubrobacter xylanophilus, both of which were previously represented only by the type strains isolated, respectively, from Japan and the United Kingdom. The new isolates were recovered from two hot springs in central Portugal after gamma irradiation of water and biofilm samples. We assessed biochemical characteristics, performed DNA-DNA hybridization, and carried out 16S rDNA sequence analysis to demonstrate that the new Rubrobacter isolates belong to the species R. radiotolerans and R. xylanophilus. We also show for the first time that the strains of R. xylanophilus and other strains of R. radiotolerans are extremely gamma radiation resistant.
在本研究中，我们对嗜温菌耐辐射红球菌（Rubrobacter radiotolerans）和嗜热菌木聚糖嗜热栖热菌（Rubrobacter xylanophilus）的新菌株进行了特性分析，这两种菌株此前仅分别由从日本和英国分离出的模式菌株所代表。新分离株是在对葡萄牙中部的两个温泉水样和生物膜样本进行伽马辐射后获得的。我们评估了生化特性，进行了DNA-DNA杂交，并开展了16S rDNA序列分析，以证明新的红球菌分离株属于耐辐射红球菌和木聚糖嗜热栖热菌这两个物种。我们还首次表明，木聚糖嗜热栖热菌的菌株以及耐辐射红球菌的其他菌株具有极强的伽马辐射抗性。
